A New Tetraphenylethylene-Derived Fluorescent Probe for Nitroreductase Detection and Hypoxic-Tumor-Cell Imaging

被引：44

作者：

You, Xue ^{[1
,2
]}

Li, Lihong ^{[1
,2
]}

Li, Xiaohua ^{[1
]}

Ma, Huimin ^{[1
,2
]}

Zhang, Guanxin ^{[1
]}

Zhang, Deqing ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Labs Organ Solids & Analyt Chem Living Biosyst, Beijing 100190, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

CHEMISTRY-AN ASIAN JOURNAL | 2016年 / 11卷 / 20期

基金：

北京市自然科学基金;

关键词：

aggregation; fluorescence; imaging agents; nitroreductase; tetraphenylethylene; AGGREGATION-INDUCED EMISSION; TURN-ON DETECTION; INFRARED FLUORESCENCE; PHOTODYNAMIC THERAPY; SELECTIVE DETECTION; CANCER-CELLS; METAL-IONS; NANOPARTICLES; ASSAY; FLUOROGENS;

D O I：

10.1002/asia.201600945

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The fluorescence detection of nitroreductase ( NTR) and evaluation of the hypoxia status of tumor cells are vital, not only for clinical diagnoses and therapy, but also for biomedical research. Herein, we report the synthesis and application of a new fluorometric "turn-on" probe for the detection of NTR ( TPE-NO2) that takes advantage of the aggregation-induced emission of tetraphenylethylene. TPE-NO2 can detect NTR at concentrations as low as 5 ng mL(-1) in aqueous solution. The detection mechanism relied on the aggregation and deaggregation of tetraphenylethylene molecules. Moreover, this fluorescent probe can be used to monitor the hypoxia status of tumor cells through the detection of endogenous NTR.

引用

页码：2918 / 2923

页数：6

共 69 条

[1] Enhanced emission and its switching in fluorescent organic nanoparticles [J].