Abscisic acid mediated proline biosynthesis and antioxidant ability in roots of two different rice genotypes under hypoxic stress

被引:51
作者
Cao, Xiaochuang [1 ]
Wu, Longlong [1 ]
Wu, Meiyan [2 ]
Zhu, Chunquan [1 ]
Jin, Qianyu [1 ]
Zhang, Junhua [1 ]
机构
[1] China Natl Rice Res Inst, State Key Lab Rice Biol, 359 Tiyuchang Rd, Hangzhou 310006, Peoples R China
[2] Yangtze Univ, Minist Educ, Engn Res Ctr Ecol & Agr Use Wetland, Hubei Collaborat Innovat Ctr Grain Ind, Jingzhou 434025, Hubei, Peoples R China
基金
中国国家自然科学基金;
关键词
Abscisic acid; Proline metabolism; Root oxidative damage; Hypoxic stress; Rice; NITRIC-OXIDE; AERENCHYMA FORMATION; OXIDATIVE STRESS; SALT-STRESS; PHYSIOLOGICAL-RESPONSES; GROWTH-RESPONSES; GENE-EXPRESSION; P5CS GENES; ACCUMULATION; OXYGEN;
D O I
10.1186/s12870-020-02414-3
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Background Abscisic acid (ABA) and proline play important roles in rice acclimation to different stress conditions. To study whether cross-talk exists between ABA and proline, their roles in rice acclimation to hypoxia, rice growth, root oxidative damage and endogenous ABA and proline accumulation were investigated in two different rice genotypes ('Nipponbare' (Nip) and 'Upland 502' (U502)). Results Compared with U502 seedlings, Nip seedlings were highly tolerant to hypoxic stress, with increased plant biomass and leaf photosynthesis and decreased root oxidative damage. Hypoxia significantly stimulated the accumulation of proline and ABA in the roots of both cultivars, with a higher ABA level observed in Nip than in U502, whereas the proline levels showed no significant difference in the two cultivars. The time course variation showed that the root ABA and proline contents under hypoxia increased 1.5- and 1.2-fold in Nip, and 2.2- and 0.7-fold in U502, respectively, within the 1 d of hypoxic stress, but peak ABA production (1 d) occurred before proline accumulation (5 d) in both cultivars. Treatment with an ABA synthesis inhibitor (norflurazon, Norf) inhibited proline synthesis and simultaneously aggravated hypoxia-induced oxidative damage in the roots of both cultivars, but these effects were reversed by exogenous ABA application. Hypoxia plus Norf treatment also induced an increase in glutamate (the main precursor of proline). This indicates that proline accumulation is regulated by ABA-dependent signals under hypoxic stress. Moreover, genes involved in proline metabolism were differentially expressed between the two genotypes, with expression mediated by ABA under hypoxic stress. In Nip, hypoxia-induced proline accumulation in roots was attributed to the upregulation of OsP5CS2 and downregulation of OsProDH, whereas upregulation of OsP5CS1 combined with downregulation of OsProDH enhanced the proline level in U502. Conclusion These results suggest that the high tolerance of the Nip cultivar is related to the high ABA level and ABA-mediated antioxidant capacity in roots. ABA acts upstream of proline accumulation by regulating the expression of genes encoding the key enzymes in proline biosynthesis, which also partly improves rice acclimation to hypoxic stress. However, other signaling pathways enhancing tolerance to hypoxia in the Nip cultivar still need to be elucidated.
引用
收藏
页数:14
相关论文
共 69 条
  • [21] Ornithine-δ-aminotransferase is essential for arginine catabolism but not for proline biosynthesis
    Funck, Dietmar
    Stadelhofer, Bettina
    Koch, Wolfgang
    [J]. BMC PLANT BIOLOGY, 2008, 8 (1)
  • [22] Exogenous calcium affects nitrogen metabolism in root-zone hypoxia-stressed muskmelon roots and enhances short-term hypoxia tolerance
    Gao, Hongbo
    Jia, Yongxia
    Guo, Shirong
    Lv, Guiyun
    Wang, Tian
    Juan, Li
    [J]. JOURNAL OF PLANT PHYSIOLOGY, 2011, 168 (11) : 1217 - 1225
  • [23] Wheat genotypes show contrasting abilities to recover from anoxia in spite of similar anoxic carbohydrate metabolism
    Goggin, Danica E.
    Colmer, Timothy D.
    [J]. JOURNAL OF PLANT PHYSIOLOGY, 2007, 164 (12) : 1605 - 1611
  • [24] Mechanisms of anoxia tolerance in plants. II. Energy requirements for maintenance and energy distribution to essential processes
    Greenway, H
    Gibbs, J
    [J]. FUNCTIONAL PLANT BIOLOGY, 2003, 30 (10) : 999 - 1036
  • [25] Relay and control of abscisic acid signaling
    Himmelbach, A
    Yang, Y
    Grill, E
    [J]. CURRENT OPINION IN PLANT BIOLOGY, 2003, 6 (05) : 470 - 479
  • [26] Arabidopsis RAP2.2: An Ethylene Response Transcription Factor That Is Important for Hypoxia Survival
    Hinz, Manuela
    Wilson, Iain W.
    Yang, Jun
    Buerstenbinder, Katharina
    Llewellyn, Danny
    Dennis, Elizabeth S.
    Sauter, Margret
    Dolferus, Rudy
    [J]. PLANT PHYSIOLOGY, 2010, 153 (02) : 757 - 772
  • [27] Removal of feedback inhibition of Δ1-pyrroline-5-carboxylate synthetase results in increased proline accumulation and protection of plants from osmotic stress
    Hong, ZL
    Lakkineni, K
    Zhang, ZM
    Verma, DPS
    [J]. PLANT PHYSIOLOGY, 2000, 122 (04) : 1129 - 1136
  • [28] Water stress-induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up-regulates the activities of antioxidant enzymes in maize leaves
    Jiang, MY
    Zhang, JH
    [J]. JOURNAL OF EXPERIMENTAL BOTANY, 2002, 53 (379) : 2401 - 2410
  • [29] Hypoxia induced non-apoptotic cellular changes during aerenchyma formation in rice (Oryza sativa L.) roots
    Joshi R.
    Shukla A.
    Mani S.C.
    Kumar P.
    [J]. Physiology and Molecular Biology of Plants, 2010, 16 (1) : 99 - 106
  • [30] Proline: a key player in plant abiotic stress tolerance
    Kaur, G.
    Asthir, B.
    [J]. BIOLOGIA PLANTARUM, 2015, 59 (04) : 609 - 619