Sanguinarine Induces Apoptosis of HT-29 Human Colon Cancer Cells via the Regulation of Bax/Bcl-2 Ratio and Caspase-9-Dependent Pathway

被引：114

作者：

Lee, Jun Sik ^{[2
]}

Jung, Won-Kyo ^{[3
,4
]}

Jeong, Myung Ho ^{[1
]}

Yoon, Taek Rim ^{[1
]}

Kim, Hyung Keun ^{[1
]}

机构：

[1] Chonnam Natl Univ, Heart Res Ctr, Chonnam Natl Univ Hosp, Cardiovasc Res Inst, Kwangju 501757, South Korea

[2] Chosun Univ, Dept Biol, Coll Nat Sci, Kwangju, South Korea

[3] Chosun Univ, Dept Marine Life Sci & Marine Life Res, Kwangju, South Korea

[4] Chosun Univ, Educ Ctr, Kwangju, South Korea

来源：

INTERNATIONAL JOURNAL OF TOXICOLOGY | 2012年 / 31卷 / 01期

关键词：

sanguinarine; human colon cancer cells; caspase; Bax/Bcl-2; ratio; DRUG-INDUCED APOPTOSIS; CYTOCHROME-C RELEASE; BCL-2 FAMILY PROTEINS; NF-KAPPA-B; ALKALOID SANGUINARINE; DNA FRAGMENTATION; CARCINOMA-CELLS; U937; CELLS; CASPASE-3; INDUCTION;

D O I：

10.1177/1091581811423845

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Sanguinarine is an alkaloid obtained from the bloodroot plant Sanguinaria canadensis and has beneficial effects on oxidative stress and inflammatory disorders. Previous reports have demonstrated that sanguinarine also exhibit anticancer properties. In the current study, we investigated the effects of sanguinarine on HT-29 human colon cancer cells. It was observed that sanguinarine treatment induces a dose-dependent increase in apoptosis of human colon cancer cells. We also investigated the effects of sanguinarine on the expression of apoptosis-associated proteins, and the results revealed that there was an increase in Bax and a decrease in B-cell lymphoma 2 (Bcl-2) protein levels. Moreover, sanguinarine treatment significantly increases the activation of caspases 3 and 9 that are the key executioners in apoptosis. Our results suggest that sanguinarine induces apoptosis of HT-29 human colon cancer cells and may have a potential therapeutic use in the treatment of human colon cancer.

引用

页码：70 / 77

页数：8

共 33 条

[1] The Bcl-2 protein family: Arbiters of cell survival [J].

Adams, JM ;

Cory, S .

SCIENCE, 1998, 281 (5381) :1322-1326

[2]

Adhami VM, 2003, CLIN CANCER RES, V9, P3176

[3] Skin tumor promotion by argemone oil/alkaloid in mice: Evidence for enhanced cell proliferation, ornithine decarboxylase, cyclooxygenase-2 and activation of MAPK/NF-κB pathway [J].

Ansari, Kausar M. ;

Das, Mukul .

FOOD AND CHEMICAL TOXICOLOGY, 2010, 48 (01) :132-138

[4] SMAC mimetics sensitize nonsteroidal anti-inflammatory drug-induced apoptosis by promoting caspase-3-mediated cytochrome c release [J].

Bank, Alexander ;

Wang, Peng ;

Du, Chunying ;

Yu, Jian ;

Zhang, Lin .

CANCER RESEARCH, 2008, 68 (01) :276-284

[5] FORMATION OF APOPTOTIC BODIES IS ASSOCIATED WITH INTERNUCLEOSOMAL DNA FRAGMENTATION DURING DRUG-INDUCED APOPTOSIS [J].

CATCHPOOLE, DR ;

STEWART, BW .

EXPERIMENTAL CELL RESEARCH, 1995, 216 (01) :169-177

[6] Role of Bcl-2 family members in caspase-3/9-dependent apoptosis during Pseudomonas aeruginosa infection in U937 cells [J].

Chai, W. S. ;

Zhu, X. M. ;

Li, S. H. ;

Fan, J. X. ;

Chen, B. Y. .

APOPTOSIS, 2008, 13 (06) :833-843

[7] Induction of necrosis and apoptosis to KB cancer cells by sanguinarine associated with reactive oxygen species production and mitochondrial membrane depolarization [J].

Chang, Mei-Chi ;

Chan, Chiu-Po ;

Wang, Ying-Jan ;

Lee, Po-Hsuen ;

Chen, Lin-I. ;

Tsai, Yi-Ling ;

Lin, Bor-Ru ;

Wang, Yin-Lin ;

Jeng, Jiiang-Huei .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 2007, 218 (02) :143-151

[8] Initiator caspases in apoptosis signaling pathways [J].

Chen, M ;

Wang, J .

APOPTOSIS, 2002, 7 (04) :313-319

[9] Sanguinarine, a benzophenanthridine alkaloid, induces apoptosis in MDA-MB-231 human breast carcinoma cells through a reactive oxygen species-mediated mitochondrial pathway [J].

Choi, Woo Young ;

Kim, Gi-Young ;

Lee, Won Ho ;

Choi, Yung Hyun .

CHEMOTHERAPY, 2008, 54 (04) :279-287

[10] Caspases: the executioners of apoptosis [J].

Cohen, GM .

BIOCHEMICAL JOURNAL, 1997, 326 :1-16

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