Hydrophilic interaction and reversed-phase ultraperformance liquid chromatography TOF-MS for serum metabonomic analysis of myocardial infarction in rats and its applications

被引：44
作者：
Tan, Guangguo [1 ,2 ]
Lou, Ziyang [1 ]
Liao, Wenting [1 ,2 ]
Dong, Xin [1 ]
Zhu, Zhenyu [1 ,2 ]
Li, Wuhong [1 ,2 ]
Chai, Yifeng [1 ,2 ]
机构：
[1] Second Mil Med Univ, Sch Pharm, Shanghai 200433, Peoples R China
[2] Shanghai Key Lab Pharmaceut Metabolite Res, Shanghai 200433, Peoples R China
来源：
MOLECULAR BIOSYSTEMS | 2012年 / 8卷 / 02期
关键词：
MASS-SPECTROMETRY; PROTEIN-KINASE; ARACHIDONIC-ACID; SYSTEMS BIOLOGY; HEART-FAILURE; FAILING HEART; PERFORMANCE; DISEASE; METABOLOMICS; ACTIVATION;
D O I：
10.1039/c1mb05324h
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An ultra performance liquid chromatography coupled to mass spectrometry-based metabonomic approach, which utilizes both reversed-performance (RP) chromatography and hydrophilic interaction chromatography (HILIC) separations, has been developed to characterize the global serum metabolic profile associated with myocardial infarction (MI). The HILIC was found necessary for a comprehensive serum metabonomic profiling, providing complementary information to RP chromatography. By combining with partial least squares discriminant analysis, 21 potential biomarkers in rat serum were identified. To further elucidate the pathophysiology of MI, related metabolic pathways have been studied. It was found that MI was closely related to disturbed sphingolipid metabolism, phospholipid catabolism, fatty acid transportation and metabolism, tryptophan metabolism, branched-chain amino acids metabolism, phenylalanine metabolism, and arginine and proline metabolism. With the presented metabonomic method, we systematically analyzed the therapeutic effects of Traditional Chinese Medicine Sini decoction (SND). The results demonstrated that SND administration could provide satisfactory effects on MI through partially regulating the perturbed metabolic pathways.
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收藏
页码：548 / 556
页数：9
相关论文
共 54 条
[1]  
Apple FS, 2001, CLIN CHEM, V47, P377
[2]   Nuclear magnetic resonance-based screening of thalassemia and quantification of some hematological parameters using chemometric methods [J].
Arjmand, Mohammad ;
Kompany-Zareh, Mohsen ;
Vasighi, Mandi ;
Parvizzadeh, Nastran ;
Zamani, Zahra ;
Nazgooei, Fereshteh .
TALANTA, 2010, 81 (4-5) :1229-1236
[3]   Protein kinase activation and myocardial ischemia/reperfusion injury [J].
Armstrong, SC .
CARDIOVASCULAR RESEARCH, 2004, 61 (03) :427-436
[4]   Phospholipid lysophosphatidylcholine as a metabolic trigger and HERG as an ionic pathway for extracellular K+ accumulation and "Short QT syndrome" in acute myocardial ischemia [J].
Bai, Yunlong ;
Wang, Jingxiong ;
Lu, Yanjie ;
Shan, Hongli ;
Yang, Baofeng ;
Wang, Zhiguo .
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2007, 20 (05) :417-428
[5]   Large-scale human metabolomics studies: A strategy for data (pre-) processing and validation [J].
Bijlsma, S ;
Bobeldijk, L ;
Verheij, ER ;
Ramaker, R ;
Kochhar, S ;
Macdonald, IA ;
van Ommen, B ;
Smilde, AK .
ANALYTICAL CHEMISTRY, 2006, 78 (02) :567-574
[6]   Investigation of Human Blood Plasma Sample Preparation for Performing Metabolomics Using Ultrahigh Performance Liquid Chromatography/Mass Spectrometry [J].
Bruce, Stephen J. ;
Tavazzi, Isabelle ;
Parisod, Veronique ;
Rezzi, Serge ;
Kochhar, Sunil ;
Guy, Philippe A. .
ANALYTICAL CHEMISTRY, 2009, 81 (09) :3285-3296
[7]   Heart metabolic disturbances in cardiovascular diseases [J].
Carvajal, K ;
Moreno-Sánchez, R .
ARCHIVES OF MEDICAL RESEARCH, 2003, 34 (02) :89-99
[8]   PROTEIN-KINASE-C DEPENDENT AND INDEPENDENT ACTIVATION OF PHOSPHOLIPASE-A2 UNDER CALCIUM IONOPHORE (A23187) EXPOSURE IN RABBIT PULMONARY ARTERIAL SMOOTH-MUSCLE CELLS [J].
CHAKRABORTI, S ;
MICHAEL, JR ;
PATRA, SK .
FEBS LETTERS, 1991, 285 (01) :104-107
[9]   PHOSPHATIDYLCHOLINE METABOLISM IN ISCHEMIC AND HYPOXIC HEARTS [J].
CHOY, PC ;
CHAN, M ;
HATCH, G ;
MAN, RYK .
MOLECULAR AND CELLULAR BIOCHEMISTRY, 1992, 116 (1-2) :53-58
[10]   Can endogenous lipid molecules serve as predictors and prognostic markers of coronary heart disease? [J].
Das, Undurti N. .
LIPIDS IN HEALTH AND DISEASE, 2008, 7 (1)
123456