Voltage-dependent and calcium-activated ion channels in the human mast cell line HMC-1

被引:0
作者
Duffy, SM [1 ]
Leyland, ML [1 ]
Conley, EC [1 ]
Bradding, P [1 ]
机构
[1] Univ Leicester, Sch Med, Inst Lung Hlth, Div Resp Med, Leicester LE1 7RH, Leics, England
关键词
human; mast cells; chloride; potassium;
D O I
暂无
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The mechanisms underlying the recruitment, differentiation, and sustained activation of mast cells in disease are likely to include modulation of ion channels. Specific Ca2+, K+, and Cl- conductances have been identified in rodent mast cells, but there are no equivalent data on human mast cells. We have used the whole-cell patch-clamp technique to characterize macroscopic ion currents in both the human mast cell line HMC-1 and human skin mast cells (HSMCs) at rest and in HMC-1 after activation with calcium ionophore. HSMCs were electrically silent at rest. In contrast, HMC-1 expressed a strong outwardly rectifying voltage-dependent Cl- conductance characteristic of ClC-4 or ClC-5 and a small inwardly rectifying K+ current not carried by the classical Kir family of K+ channels. Calcium ionophore induced the appearance of outwardly rectifying Ca2+-activated Cl- and K+ currents, while hypotonicity induced another outwardly rectifying conductance typical of ClC-3. Reverse transcription-PCRs confirmed that mRNAs for the voltage-dependent Cl- channels ClC-3 and -5 were expressed. This is the first definitive description of a ClC-4/5-like current in a native leukocyte. We suggest that this current may contribute to the malignant phenotype while the Ca2+-activated K+ and Cl- currents may he involved in cell activation.
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页码:233 / 240
页数:8
相关论文
共 35 条
[1]   COMMITMENT TO DIFFERENTIATION OF MURINE ERYTHROLEUKEMIA-CELLS INVOLVES A MODULATED PLASMA-MEMBRANE DEPOLARIZATION THROUGH CA-2+-ACTIVATED K+-CHANNELS [J].
ARCANGELI, A ;
RICUPERO, L ;
OLIVOTTO, M .
JOURNAL OF CELLULAR PHYSIOLOGY, 1987, 132 (03) :387-400
[2]  
Baghestanian M, 1997, THROMB HAEMOSTASIS, V77, P577
[3]  
BRADDING P, 1995, IMMUNOPHARMACOLOGY R, P53
[4]   ESTABLISHMENT OF AN IMMATURE MAST-CELL LINE FROM A PATIENT WITH MAST-CELL LEUKEMIA [J].
BUTTERFIELD, JH ;
WEILER, D ;
DEWALD, G ;
GLEICH, GJ .
LEUKEMIA RESEARCH, 1988, 12 (04) :345-355
[5]   Molecular identification of a volume-regulated chloride channel [J].
Duan, D ;
Winter, C ;
Cowley, S ;
Hume, JR ;
Horowitz, B .
NATURE, 1997, 390 (6658) :417-421
[6]   CA2+ AND MN2+ INFLUX THROUGH RECEPTOR-MEDIATED ACTIVATION OF NONSPECIFIC CATION CHANNELS IN MAST-CELLS [J].
FASOLATO, C ;
HOTH, M ;
MATTHEWS, G ;
PENNER, R .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (07) :3068-3072
[7]  
FISHER SE, 1994, HUM MOL GENET, V3, P2053
[8]   Electrical and hemodynamic correlates of the maximal rate of pressure increase in the human left ventricle [J].
Fried, AG ;
Parker, AB ;
Newton, GE ;
Parker, JD .
JOURNAL OF CARDIAC FAILURE, 1999, 5 (01) :8-16
[9]  
Gruber AD, 1999, AM J PHYSIOL-CELL PH, V276, pC1261
[10]   Genomic cloning, molecular characterization, and functional analysis of human CLCA1, the first human member of the family of Ca2+-activated Cl- channel proteins [J].
Gruber, AD ;
Elble, RC ;
Ji, HL ;
Schreur, KD ;
Fuller, CM ;
Pauli, BU .
GENOMICS, 1998, 54 (02) :200-214