Synthesis and Incorporation of Quaternary Ammonium Silane Antimicrobial into Self-Crosslinked Type I Collagen Scaffold: A Hybrid Formulation for 3D Printing

被引：5

作者：

Bapat, Ranjeet Ajit ^{[1
]}

Muthusamy, Senthil Kumar ^{[2
]}

Sidhu, Preena ^{[1
]}

Mak, Kit-Kay ^{[3
]}

Parolia, Abhishek ^{[1
]}

Pichika, Mallikarjuna Rao ^{[3
]}

Seow, Liang Lin ^{[1
]}

Tong, Coo ^{[2
]}

Daood, Umer ^{[1
]}

机构：

[1] Int Med Univ Kuala Lumpur, Div Restorat Dent, Sch Dent, 126 Jalan Jalil Perkasa 19, Kuala Lumpur 57000, Malaysia

[2] Natl Univ Singapore, Fac Dent, Oral Sci, 21 Lower Kent Ridge Rd, Singapore 119077, Singapore

[3] Int Med Univ Kuala Lumpur, Sch Pharm, 126 Jalan Jalil Perkasa 19, Kuala Lumpur 57000, Malaysia

来源：

MACROMOLECULAR BIOSCIENCE | 2022年 / 22卷 / 03期

关键词：

3D printing; antimicrobial; collagen scaffold; membranes; quaternary ammonium silane; LINKING; MEMBRANES; REGENERATION; RIBOFLAVIN; ULTRAVIOLET; DISEASE; TPGS;

D O I：

10.1002/mabi.202100326

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Novel 3D-biomaterial scaffold is constructed having a combination of a new quaternary ammonium silane (k21) antimicrobial impregnated in 3D collagen printed scaffolds cross linked with Riboflavin in presence of d-alpha-tocopheryl poly(ethyleneglycol)-1000-succinate. Groups of "0.1% and 0.2% k21", and "0.1% and 0.2% Chlorhexidine (CHX)" are prepared. k21/CHX with neutralized collagen is printed with BioX. Riboflavin is photo-activated and examined using epifluorescence for Aggregatibacter actinomycetemcomitans (7-days). Collagen is examined using TEM and measured for porosity, and shape-fitting. Raman and tandem mass/solid-state are performed with molecular-docking and circular-dichroism. X-ray diffractions, rheological tests, contact angle, and ninhydrin assay are conducted. k21 samples demonstrated collagen aggregates while 0.1% CHX and 0.2% CHX showed irregularities. Porosity of control and "0.1% and 0.2% k21" scaffolds show no differences. Low contact angle, improved elastic-modulus, rigidity, and smaller strain in k21 groups are seen. Bacteria are reduced and strong organic intensities are seen in k21 scaffolds. Simulation shows hydrophobicity/electrostatic interaction. Crosslinking is observed in 0.2% CHX/79% and 0.2% k21/80%. Circular dichroism for k21 are suggestive of triple helix. XRD patterns appear at d = 5.97, 3.03, 2.78, 2.1, and 2.90 A degrees. 3D-printing of collagen impregnated with quaternary ammonium silane produces a promising scaffold with antimicrobial potency and structural stability.

引用

页数：16

共 53 条

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