Function of small GTPase Rho3 in regulating growth, conidiation and virulence of Botrytis cinerea

被引：33

作者：

An, Bang ^{[1
,2
]}

Li, Boqiang ^{[1
]}

Qin, Guozheng ^{[1
]}

Tian, Shiping ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China

[2] Univ Chinese Acad Sci, Being 100049, Peoples R China

来源：

FUNGAL GENETICS AND BIOLOGY | 2015年 / 75卷

基金：

中国国家自然科学基金;

关键词：

Rho3; Botrytis cinerea; Virulence; ROS; Mitochondria; GENERATING NADPH OXIDASES; POLARIZED CELL-GROWTH; ASPERGILLUS-NIDULANS; MITOCHONDRIA; PATHOGENICITY; METABOLISM; PROTEINS; DISEASE; GERMINATION; ACTIVATION;

D O I：

10.1016/j.fgb.2015.01.007

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Small GTPases of the Rho family play an important role in regulating biological processes in fungi. In this study, we mainly investigated the biological functions of Rho3 in Botrytis cinerea, and found that deletion of the rho3 from B. cinerea significantly suppressed vegetative growth and conidiation, reduced appressorium formation and decreased virulence. Microscopy analysis revealed that the distance between septa was increased in the Delta rho3 mutant. In addition, mitochondria were suggested to be the main sources of intracellular reactive oxygen species (ROS) in B. cinerea based on dual staining with 2',7'-dichlorodihydro-fluorescein diacetate and MitoTracker orange. The Arho3 mutant showed less accumulation of ROS in the hyphae tips compared to the WT strain of B. cinerea. These results provide the novel evidence to ascertain the function of small GTPase Rho3 in regulating growth, conidiation and virulence of B. cinerea. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：46 / 55

页数：10

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