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Micelle to solvent stacking of organic cations in micellar electrokinetic chromatography with sodium dodecyl sulfate
被引:35
|作者:
Quirino, Joselito P.
[1
,2
]
Aranas, Agnes T.
[1
,2
]
机构:
[1] Univ Tasmania, Sch Chem, Australian Ctr Res Separat Sci ACROSS, Hobart, Tas 7001, Australia
[2] Ateneo Manila Univ, Loyola Sch, Sch Sci & Engn, Dept Chem, Quezon City 1108, Metro Manila, Philippines
基金:
澳大利亚研究理事会;
关键词:
Micellar electrokinetic chromatography;
Drugs;
Herbicides;
Micelle to solvent stacking;
Analyte focusing by micelle collapse;
Sweeping;
CAPILLARY-ZONE-ELECTROPHORESIS;
ONLINE SAMPLE PRECONCENTRATION;
DYNAMIC PH JUNCTION;
SELECTIVE EXHAUSTIVE INJECTION;
TRANSIENT ISOTACHOPHORESIS;
SENSITIVITY ENHANCEMENT;
COLLAPSE;
DRUGS;
CZE;
SEPARATION;
D O I:
10.1016/j.chroma.2011.08.028
中图分类号:
Q5 [生物化学];
学科分类号:
071010 ;
081704 ;
摘要:
The on-line sample concentration technique, micelle to solvent stacking (MSS), was studied for small organic cations (quaternary ammonium herbicides, beta-blocker drugs, and tricyclic antidepressant drugs) in reversed migration micellar electrokinetic chromatography. Electrokinetic chromatography was carried out in fused silica capillaries with a background solution of sodium dodecyl sulfate (SDS) in a low pH phosphate buffer. MSS was performed using anionic SDS micelles in the sample solution for analyte transport and methanol or acetonitrile as organic solvent in the background solution for analyte effective electrophoretic mobility reversal. The solvent also allowed for the separation of the analyte test mixtures. A model for focusing and separation was developed and the mobility reversal that involved micelle collapse was experimentally verified. The effect of analyte retention factor was observed by changing the % organic solvent in the background solution or the concentration of SDS in the sample matrix. With an injection length of 31.9 cm (77% of effective capillary length) for the 7 test drugs, the LODs (S/N = 3) of 5-14 ng/mL were 101-346-fold better when compared to typical injection. The linearity (R(2), range = 0.025-0.8 mu g/mL), intraday and interday repeatability (%RSD, n = 10) were >= 0.988, <6.0% and <8.5%, respectively. In addition, analysis of spiked urine samples after 10-fold dilution with the sample matrix yielded LODs = 0.02-0.10 mu g/mL. These LODs are comparable to published electrophoretic methods that required off-line sample concentration. However, the practicality of the technique for more complex samples will rely on dedicated sample preparation schemes. (C) 2011 Elsevier B.V. All rights reserved.
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页码:7377 / 7383
页数:7
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