Towards industrially-feasible delignification and pitch removal by treating paper pulp with Myceliophthora thermophila laccase and a phenolic mediator

被引：63

作者：

Babot, Esteban D. ^{[1
]}

Rico, Alejandro ^{[1
]}

Rencoret, Jorge ^{[1
]}

Kalum, Lisbeth ^{[2
]}

Lund, Henrik ^{[2
]}

Romero, Javier ^{[3
]}

del Rio, Jose C. ^{[1
]}

Martinez, Angel T. ^{[4
]}

Gutierrez, Ana ^{[1
]}

机构：

[1] CSIC, Inst Recursos Nat & Agrobiol Sevilla, E-41080 Seville, Spain

[2] Novozymes AS, DK-2880 Bagsvaerd, Denmark

[3] ENCE, E-36080 Pontevedra, Spain

[4] CSIC, Ctr Invest Biol, E-28040 Madrid, Spain

来源：

BIORESOURCE TECHNOLOGY | 2011年 / 102卷 / 12期

关键词：

Laccase; Methyl syringate; Pitch deposits; Pulp bleaching; Syringaldehyde; GAS CHROMATOGRAPHY/MASS SPECTROMETRY; LIPOPHILIC EXTRACTIVES; NATURAL MEDIATORS; KRAFT PULP; LIGNIN STRUCTURES; EUCALYPT PULP; SYSTEM; OXIDATION; DEPOSITS; STEROLS;

D O I：

10.1016/j.biortech.2011.03.100

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The ability of two natural phenols to act as mediators of the recombinant Myceliophthora thermophila laccase (MtL) in eucalypt-pulp delignification was investigated. After alkaline peroxide extraction, the properties of the enzymatically-treated pulps improved with respect to the control. The pulp brightness increased (3.1 points) after the enzymatic treatment with MtL alone, but the highest improvements were obtained after the MtL treatment using syringaldehyde (4.7 points) and especially methyl syringate (8.3 points) as mediators. Likewise, a decrease in kappa number up to 2.7 points was obtained after the MtL-methyl syringate treatment, followed by decreases of 1.4 and 0.9 points after the treatments with MtL-syringaldehyde and MtL alone, respectively. On the other hand, removal of the main lipophilic extractives present in eucalypt pulp was observed after the above laccase-mediator treatments. Finally, the doses of both MtL and methyl syringate were reduced, and results compatible with industrial implementation were obtained. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：6717 / 6722

页数：6

共 35 条

[1]

Bajpai P, 2006, BIOTECHNOL ANN REV, V12, P349, DOI 10.1016/S1387-2656(06)12010-4

[2] Characterization of the gene encoding an extracellular laccase of Myceliophthora thermophila and analysis of the recombinant enzyme expressed in Aspergillus oryzae [J].

Berka, RM ;

Schneider, P ;

Golightly, EJ ;

Brown, SH ;

Madden, M ;

Brown, KM ;

Halkier, T ;

Mondorf, K ;

Xu, F .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (08) :3151-3157

[3]

BERKA RM, 1999, Patent No. 5981243

[4]

Bourbonnais R, 1996, TAPPI J, V79, P199

[5] OXIDATION OF NONPHENOLIC SUBSTRATES - AN EXPANDED ROLE FOR LACCASE IN LIGNIN BIODEGRADATION [J].

BOURBONNAIS, R ;

PAICE, MG .

FEBS LETTERS, 1990, 267 (01) :99-102

[6]

CALL HP, 1994, Patent No. 9429510

[7] Efficient bleaching of non-wood high-quality paper pulp using laccase-mediator system [J].

Camarero, S ;

García, O ;

Vidal, T ;

Colom, J ;

del Río, JC ;

Gutiérrez, A ;

Gras, JM ;

Monje, R ;

Martínez, MJ ;

Martínez, AT .

ENZYME AND MICROBIAL TECHNOLOGY, 2004, 35 (2-3) :113-120

[8] Paper pulp delignification using laccase and natural mediators [J].

Camarero, Susana ;

Ibarra, David ;

Martinez, Angel T. ;

Romero, Javier ;

Gutierrez, Ana ;

del Rio, Jos C. .

ENZYME AND MICROBIAL TECHNOLOGY, 2007, 40 (05) :1264-1271

[9] Identification of residual lignin markers in eucalypt kraft pulps by Py-GC/MS [J].

del Río, JC ;

Gutiérrez, A ;

Romero, J ;

Martínez, MJ ;

Martínez, AT .

JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS, 2001, 58 :425-439

[10] A new approach to the biobleaching of flax pulp with laccase using natural mediators [J].

Fillat, Amanda ;

Colom, Josep F. ;

Vidal, Teresa .

BIORESOURCE TECHNOLOGY, 2010, 101 (11) :4104-4110

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