Ethanol precipitation and ultrafiltration of inulinases from Kluyveromyces marxianus

被引：35

作者：

Golunski, Simone ^{[1
]}

Astolfi, Viviane ^{[1
]}

Carniel, Naira ^{[1
]}

de Oliveira, Debora ^{[1
]}

Di Luccio, Marco ^{[1
]}

Mazutti, Marcio A. ^{[1
]}

Treichel, Helen ^{[1
]}

机构：

[1] Univ Reg Integrada Alto Uruguai & Missoes, Dept Food Engn, BR-99700000 Erechim, RS, Brazil

来源：

SEPARATION AND PURIFICATION TECHNOLOGY | 2011年 / 78卷 / 03期

关键词：

Inulinase; Precipitation; Ultrafiltration; SOLID-STATE FERMENTATION; LIQUID-LIQUID-EXTRACTION; SUGARCANE BAGASSE; BETA-XYLOSIDASE; PURIFICATION; SEPARATION; PROTEINS; PARAMETERS;

D O I：

10.1016/j.seppur.2011.02.019

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Inulinase (EC 3.2.1.7) produced by Kluyveromyces marxianus NRRL Y-7571 by solid state fermentation of sugarcane bagasse was purified from culture broth by ethanol precipitation and ultrafiltration. The effects of the concentration of ethanol and the rate of addition of ethanol to the crude extract on the purification yield and purification factor were assessed using the experimental design technique. Precipitation caused an activation of enzyme and allowed purification factor up to 2-fold. After precipitation with ethanol 55% (v/v) and a flow rate of 10 mL/min, followed by ultrafiltration with a 100 kDa membrane, the inulinase could be purified by a factor of 5.5-fold with 86.1% yield. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：261 / 265

页数：5

共 33 条

[1] Extraction of inulinase obtained by solid state fermentation of sugarcane bagasse by Kluyveromyces marxianus NRRL Y-7571 [J].

Bender, Joao Paulo ;

Mazutti, Marcio Antonio ;

Di Luccio, Marco ;

Treichel, Helen .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2008, 149 (03) :195-203

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] Purification and characterisation of exo- and endo-inulinase from Aspergillus ficuum JNSP5-06 [J].

Chen, Han-Qing ;

Chen, Xiao-Ming ;

Li, Yin ;

Wang, Jing ;

Jin, Zheng-Yu ;

Xu, Xue-Ming ;

Zhao, Jian-Wei ;

Chen, Tian-Xiang ;

Xie, Zheng-Jun .

FOOD CHEMISTRY, 2009, 115 (04) :1206-1212

[4] Liquid-liquid extraction of xylitol dehydrogenase from Candida guilliermondii homogenate by reversed micelles [J].

Cortez, EV ;

Pessoa, A ;

Felipe, MDD ;

Roberto, IC ;

Vitolo, M .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2004, 807 (01) :55-60

[5] Xylanase and β-xylosidase separation by fractional precipitation [J].

Cortez, EV ;

Pessoa, A .

PROCESS BIOCHEMISTRY, 1999, 35 (3-4) :277-283

[6] Purification and characterization of transglutaminase from a newly isolated Streptomyces hygroscopicus [J].

Cui, Li ;

Du, Guocheng ;

Zhang, Dongxu ;

Liu, He ;

Chen, Jian .

FOOD CHEMISTRY, 2007, 105 (02) :612-618

[7] Ultrafiltration within downstream processing: some process design considerations [J].

Darnon, E ;

Morin, E ;

Belleville, MP ;

Rios, GM .

CHEMICAL ENGINEERING AND PROCESSING-PROCESS INTENSIFICATION, 2003, 42 (04) :299-309

[8] Sucrose hydrolysis by thermostable immobilized inulinases from Aspergillus ficuum [J].

Ettalibi, M ;

Baratti, JC .

ENZYME AND MICROBIAL TECHNOLOGY, 2001, 28 (7-8) :596-601

[9]

Galaev I.Y., 2001, MEMBRANE SEPARATIONS

[10] Protein purification by ultrafiltration with pre-treated membrane [J].

Ghosh, R ;

Cui, ZF .

JOURNAL OF MEMBRANE SCIENCE, 2000, 167 (01) :47-53

← 1 2 3 4 →