Combined in vivo multiphoton and CARS imaging of healthy and disease-affected human skin

被引：49

作者：

Breunig, Hans Georg ^{[1
]}

Bueckle, Rainer ^{[1
]}

Kellner-Hoefer, Marcel ^{[1
]}

Weinigel, Martin ^{[1
]}

Lademann, Juergen ^{[2
]}

Sterry, Wolfram ^{[2
]}

Koenig, Karsten ^{[1
,3
]}

机构：

[1] JenLab GmbH, D-66123 Saarbrucken, Germany

[2] Charite, Dept Dermatol Venerol & Allergol, D-10117 Berlin, Germany

[3] Univ Saarland, Dept Biophoton & Laser Technol, D-66123 Saarbrucken, Germany

来源：

MICROSCOPY RESEARCH AND TECHNIQUE | 2012年 / 75卷 / 04期

关键词：

CARS tomography; non-linear microscopy; skin imaging; multiphoton imaging; RAMAN SCATTERING MICROSCOPY; EXCITATION FLUORESCENCE MICROSCOPY; EX-VIVO; 2ND-HARMONIC GENERATION; SPECTROSCOPIC ANALYSIS; RESOLUTION; CELLS; VISUALIZATION; TOMOGRAPHY; PROTEINS;

D O I：

10.1002/jemt.21082

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

We present combined epi-coherent anti-Stokes Raman scattering (CARS) and multiphoton imaging with both chemical discrimination and subcellular resolution on human skin invivo. The combination of both image modalities enables label-free imaging of the autofluorescence of endogenous fluorophores by two-photon excited fluorescence, as well as imaging of the distribution of intercellular lipids, topically applied substances and water by CARS. As an example for medical imaging, we investigated healthy and psoriasis-affected human skin with both image modalities in vivo and found indications for different lipid distributions on the cellular level. Microsc. Res. Tech., 2011. (C) 2011 Wiley-Liss, Inc.

引用

页码：492 / 498

页数：7

共 46 条

[1] In vivo optical monitoring of tissue pathologies and diseases with vibrational contrast [J].