Comparing a two-dimensional liquid chromatography with a quick, easy, cheap, effective, rugged, and safe protocol-based liquid chromatography method for matrix removal in pesticide analysis using time-of-flight mass spectrometry

被引:7
作者
Muehlwald, Sandra [1 ]
Meyburg, Nina [1 ]
Rohn, Sascha [2 ]
Buchner, Nadja [1 ]
机构
[1] Fed Off Consumer Protect & Food Safety, Mauerstr 39-42, D-10117 Berlin, Germany
[2] Univ Hamburg, Hamburg Sch Food Sci, Inst Food Chem, Grindelallee 117, D-20146 Hamburg, Germany
关键词
2D-LC; Two-dimensional liquid chromatography; HILIC; QuEChERS; Matrix fingerprinting; Pesticides; RESIDUE ANALYSIS; MULTIRESIDUE METHOD; CLEANUP; FRUIT;
D O I
10.1016/j.chroma.2020.461153
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In this study, a clean-up approach using a two-dimensional liquid chromatography (2D-LC) consisting of a hydrophilic interaction liquid chromatography column and a reversed phase column was investigated. A fully automated 2D-LC system was used and compared with a traditional quick, easy, cheap, effective, rugged, and safe (QuEChERS) liquid chromatography (QuE-LC) method. The comparison was based on the results of a validation of selected analytes. It was investigated whether the detectability of analytes could be improved by the use of the 2D-LC. On the basis of these results, the relative detection rates were determined for every matrix. By means of those detection rates, the matrices were categorized regarding their complexity. Furthermore, the applicability of the 2D-LC was tested by participation in the European Proficiency Test in Fruits and Vegetables Screening Methods. In order to evaluate the separation and the elution profile of matrix components, multivariate data analysis was applied. For this purpose, ten matrices were processed in accordance to a QuEChERS protocol and the protocol for 2D-LC analysis. Moreover, the reagent blanks of the corresponding matrix were processed and analyzed by QuE-LC and 2D-LC. The results allowed evaluating the number of detected compounds for both methods. Additionally, the influence of compounds originating from reagent blanks can be estimated. In general, less compounds could be detected when 2D-LC was used. Especially, these were very polar compounds and compounds with m/z values >1500. These compounds seem to originate primarily from the used reagents especially from the citrate salts. However, the most of these compounds could be separated and were not detectable any more when 2D-LC was used. The results of the comparison based on validation and participation in the European Proficiency Test also show a better detectability for the most analytes with 2D-LC. (C) 2020 Elsevier B.V. All rights reserved.
引用
收藏
页数:12
相关论文
共 38 条
  • [1] Agilent Technologies, DAT PREP HIGH RES LC
  • [2] Agilent Technologies, 2019, MASSH PROF SOFTW QUI
  • [3] Aguera A., 2017, APPL HIGH RESOLUTION, P59
  • [4] Anastassiades M, 2003, J AOAC INT, V86, P412
  • [5] Anastassiades M., 2007, Pesticide Chemistry: Crop protection, public health, environmental safety, DOI 10.1002/
  • [6] Matrix Effects and Interferences of Different Citrus Fruit Coextractives in Pesticide Residue Analysis Using Ultrahigh-Performance Liquid Chromatography-High-Resolution Mass Spectrometry
    Besil, Natalia
    Cesio, Veronica
    Heinzen, Horacio
    Fernandez-Alba, Amadeo R.
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2017, 65 (23) : 4819 - 4829
  • [7] Botitsi H., 2017, Applications in high resolution mass spectrometry, P83, DOI [10.1016/B978-0-12-809464-8.00004-X, DOI 10.1016/B978-0-12-809464-8.00004-X]
  • [8] Systematic and comprehensive strategy for reducing matrix effects in LC/MS/MS analyses
    Chambers, Erin
    Wagrowski-Diehl, Diane M.
    Lu, Ziling
    Mazzeo, Jeffrey R.
    [J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2007, 852 (1-2): : 22 - 34
  • [9] European Comission Directorate General Health and Food Safety, SANTE118132017 EUR C
  • [10] European Union Laboratory for Pesticide Residues in Fruits and Vegetables, SCREEN METH 10 PEST