Tibet plateau probiotic mitigates chromate toxicity in mice by alleviating oxidative stress in gut microbiota

被引:41
作者
Feng, Pengya [1 ]
Ye, Ze [1 ]
Han, Huawen [1 ]
Ling, Zhenmin [1 ]
Zhou, Tuoyu [1 ]
Zhao, Shuai [1 ]
Virk, Amanpreet Kaur [2 ]
Kakade, Apurva [1 ]
Abomohra, Abd El-Fatah [3 ]
El-Dalatony, Marwa M. [4 ]
Salama, EI-Sayed [5 ]
Liu, Pu [1 ]
Li, Xiangkai [1 ]
机构
[1] Lanzhou Univ, Minist Educ, Key Lab Cell Act & Stress Adaptat, Sch Life Sci, Lanzhou 730000, Gansu, Peoples R China
[2] Shoolini Univ Biotechnol & Management Sci, Fac Appl Sci & Biotechnol, Solan 173212, Himachal Prades, India
[3] Tanta Univ, Fac Sci, Bot Dept, Tanta 31527, Egypt
[4] Hanyang Univ, Dept Earth Resources & Environm Engn, Seoul 04763, South Korea
[5] Lanzhou Univ, Dept Occupat & Environm Hlth, Sch Publ Hlth, Lanzhou 730000, Gansu, Peoples R China
关键词
PEDIOCOCCUS-ACIDILACTICI; WASTE-WATER; INFLAMMATION; REDUCTION; BACTERIA; CR(VI); BIOAUGMENTATION; COLONIZATION; RUBRERYTHRIN; PROTECTION;
D O I
10.1038/s42003-020-0968-3
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Feng et al. show that colonization of mice with Pediococcus acidilactici strain BT36 reduces chromate accumulation in the liver, which is associated with changes in expression of a Cr(VI)-resistance gene in the gut microbiota. This study suggests that BT36 regulates the gut microbiota in response to chrome exposure, protecting mice against chromate toxicity. Heavy metal contamination in food endangers human health. Probiotics can protect animals and human against heavy metals, but the detoxification mechanism has not been fully clarified. Here, mice were supplemented with Pediococcus acidilactici strain BT36 isolated from Tibetan plateau yogurt, with strong antioxidant activity but no chromate reduction ability for 20 days to ensure gut colonization. Strain BT36 decreased chromate accumulation, reduced oxidative stress, and attenuated histological damage in the liver of mice. 16S rRNA and metatranscriptome sequencing analysis of fecal microbiota showed that BT36 reversed Cr(VI)-induced changes in gut microbial composition and metabolic activity. Specifically, BT36 recovered the expressions of 788 genes, including 34 inherent Cr remediation-relevant genes. Functional analysis of 10 unannotated genes regulated by BT36 suggested the existence of a new Cr(VI)-reduction gene in the gut microbiota. Thus, BT36 can modulate the gut microbiota in response to Cr(VI) induced oxidative stress and protect against Cr toxicity.
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页数:12
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