Metabolic profile of 2-(2-hydroxypropanamido) benzoic acid in rats by ultra high performance liquid chromatography combined with Fourier transform ion cyclotron resonance mass spectrometry

被引：13

作者：

Guan, Jiao ^{[1
,2
]}

Zhang, Qili ^{[1
]}

Rong, Rong ^{[1
]}

Han, Fei ^{[1
]}

Zhu, Heyun ^{[1
,2
]}

Zhao, Yunli ^{[1
]}

Song, Aihua ^{[1
]}

Yu, Zhiguo ^{[1
]}

机构：

[1] Shenyang Pharmaceut Univ, Sch Pharm, Shenyang 110016, Peoples R China

[2] Jilin Med Coll, Sch Pharm, Fengman Dist 132013, Jilin, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2015年 / 993卷

基金：

中国国家自然科学基金;

关键词：

2-(2-hydroxypropanamido) benzoic acid; UHPLC-FT-ICR-MS; Metabolism; Identification; Rat; DRUG-METABOLISM; MISCELLANEOUS MECHANISMS; ANTIVIRAL ACTIVITIES; MARINE PHARMACOLOGY; NERVOUS SYSTEMS; IN-VITRO; INFLAMMATION; IDENTIFICATION; ASPIRIN; IMMUNE;

D O I：

10.1016/j.jchromb.2015.04.039

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An ultra high performance liquid chromatography combined with Fourier transform ion cyclotron resonance mass spectrometry (UHPLC-FT-ICR-MS) method was developed to investigate the in vivo metabolism of 2-(2-hydroxypropanamido) benzoic acid (HPABA), a marine-derived anti-inflammatory drug, for the first time. Plasma, urine, feces and bile samples were collected from male and female rats after a single intragastric administration of HPABA at a dose of 100 mg/kg. Besides the parent drug, a total of 13 metabolites (3 phase land 10 phase II metabolites) were detected and tentatively identified through comparing their mass spectrometry profiles with those of HPABA. Results demonstrated that metabolic pathways of HPABA in rats included decarboxylation, hydroxylation, dehydrogenation, glucuronidation, glycine conjugation and N-acetyl conjugation. in summary, this work provided valuable information regarding the metabolism of HPABA in rats, which would contribute to better understanding of its safety and mechanism of action. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：60 / 68

页数：9

共 39 条

[1] Modulation of LPS induced inflammatory response by Lawsonyl monocyclic terpene from the marine derived Streptomyces sp. [J].

Ali, Asif ;

Khajuria, Anamika ;

Sidiq, Tabasum ;

Kumar, Ashok ;

Thakur, Narsinh L. ;

Naik, Deepak ;

Vishwakarma, Ram A. .

IMMUNOLOGY LETTERS, 2013, 150 (1-2) :79-86

[2] Cunninghamella - A microbial model for drug metabolism studies - A review [J].

Asha, Sepuri ;

Vidyavathi, Maravajhala .

BIOTECHNOLOGY ADVANCES, 2009, 27 (01) :16-29

[3] A calculated response: control of inflammation by the innate immune system [J].

Barton, Gregory M. .

JOURNAL OF CLINICAL INVESTIGATION, 2008, 118 (02) :413-420

[4] Polysaccharide isolated from Agardhiella ramosissima: Chemical structure and anti-inflammation activity [J].

Batista, Jalles A. ;

Dias, Eulina G. N. ;

Brito, Tarcisio V. ;

Prudencio, Rafael S. ;

Silva, Renan O. ;

Ribeiro, Ronaldo A. ;

Souza, Marcellus Henrique L. P. ;

de Paula, Regina C. M. ;

Feitosa, Judith P. A. ;

Chaves, Luciano S. ;

Melo, Marcia R. S. ;

Freitas, Ana L. P. ;

Medeiros, Jand-Venes R. ;

Barbosa, Andre L. R. .

CARBOHYDRATE POLYMERS, 2014, 99 :59-67

[5]

Bigler J, 2001, CANCER RES, V61, P3566

[6] Inflammatory Disease Processes and Interactions with Nutrition [J].

Calder, P. C. ;

Albers, R. ;

Antoine, J. -M. ;

Blum, S. ;

Bourdet-Sicard, R. ;

Ferns, G. A. ;

Folkerts, G. ;

Friedmann, P. S. ;

Frost, G. S. ;

Guarner, F. ;

Lovik, M. ;

Macfarlane, S. ;

Meyer, P. D. ;

M'Rabet, L. ;

Serafini, M. ;

van Eden, W. ;

van Loo, J. ;

Dias, W. Vas ;

Vidry, S. ;

Winklhofer-Roob, B. M. ;

Zhao, J. .

BRITISH JOURNAL OF NUTRITION, 2009, 101 :S1-+

[7] Fatty acids and inflammation: The cutting edge between food and pharma [J].

Calder, Philip C. .

EUROPEAN JOURNAL OF PHARMACOLOGY, 2011, 668 :S50-S58

[8] Evaluation of anti-inflammatory and mechanism of action of extract of Macrosiphonia longiflora (Desf.) Mull. Arg [J].

da Silva, Anisio Onorio ;

Alves, Aurea Damaceno ;

Tavares de Almeida, Danielle Ayr ;

Balogun, Sikiru Olaitan ;

de Oliveira, Ruberlei Godinho ;

Aguiar, Aline Aires ;

Soares, Ilsamar Mendes ;

Marson-Ascencio, Poliana Guerino ;

Ascencio, Sergio Donizeti ;

de Oliveira Martins, Domingos Tabajara .

JOURNAL OF ETHNOPHARMACOLOGY, 2014, 154 (02) :319-329

[9] Identification of poliumoside metabolites in rat feces by high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry [J].

Deng, Rui ;

Xu, Yunhui ;

Feng, Feng ;

Liu, Wenyuan .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2014, 969 :285-296

[10] Enhanced chemopreventive effects of a hydrogen sulfide-releasing anti-inflammatory drug (ATB-346) in experimental colorectal cancer [J].

Elsheikh, Wagdi ;

Blackler, Rory W. ;

Flannigan, Kyle L. ;

Wallace, John L. .

NITRIC OXIDE-BIOLOGY AND CHEMISTRY, 2014, 41 :131-137

← 1 2 3 4 →