Rapid and Sensitive Detection of Mycobacterium ulcerans by Use of a Loop-Mediated Isothermal Amplification Test

被引：31

作者：

Njiru, Zablon K. ^{[1
]}

Yeboah-Manu, Dorothy ^{[2
]}

Stinear, Timothy P. ^{[3
]}

Fyfe, Janet A. M. ^{[4
]}

机构：

[1] Univ Queensland, Sch Vet Sci, Gatton, Qld, Australia

[2] Univ Ghana, Noguchi Mem Inst Med Res, Legon, Ghana

[3] Univ Melbourne, Dept Microbiol & Immunol, Parkville, Vic 3052, Australia

[4] Victorian Infect Dis Reference Lab, Mycobacterium Reference Lab, Melbourne, Vic, Australia

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 05期

关键词：

HUMAN AFRICAN TRYPANOSOMIASIS; POLYMERASE-CHAIN-REACTION; BURULI ULCER; DISEASE; DIAGNOSIS; GHANA; ASSAY; LAMP;

D O I：

10.1128/JCM.06460-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This work reports the design and evaluation of a rapid loop-mediated isothermal amplification test for detecting Mycobacterium ulcerans DNA based on the multicopy insertion sequence IS2404. The test is robust and specific with a detection limit equivalent to 20 copies of the target sequence (0.01 to 0.1 genome). The test has potential for the diagnosis of Buruli ulcer under field conditions.

引用

页码：1737 / 1741

页数：5

共 21 条

[1] Assessing water-related risk factors for Buruli ulcer: A case-control study in Ghana [J].

Aiga, H ;

Amano, T ;

Cairncross, S ;

Domako, JA ;

Nanas, OK ;

Coleman, S .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2004, 71 (04) :387-392

[2] Socioeconomic implications of Buruli ulcer in Ghana: A three-year review [J].

Asiedu, K ;

Etuaful, S .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1998, 59 (06) :1015-1022

[3]

Cobb B, 1994, NUCLEIC ACIDS RES, V22, P3301

[4] Mycobacterium ulcerans disease (Buruli ulcer) in rural hospital, southern Benin, 1997-2001 [J].

Debacker, M ;

Aguiar, J ;

Steunou, C ;

Zinsou, C ;

Meyers, WM ;

Guédénon, A ;

Scott, JT ;

Dramaix, M ;

Portaels, F .

EMERGING INFECTIOUS DISEASES, 2004, 10 (08) :1391-1398

[5]

Dominguez M, 2010, INT J TUBERC LUNG D, V14, P1468

[6] Applications of Loop-Mediated Isothermal DNA Amplification [J].

Fu, Shijun ;

Qu, Guanggang ;

Guo, Shijin ;

Ma, Lin ;

Zhang, Na ;

Zhang, Songlin ;

Gao, Sanyang ;

Shen, Zhiqiang .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2011, 163 (07) :845-850

[7] Development and application of two multiplex real-time PCR assays for the detection of Mycobacterium ulcerans in clinical and environmental samples [J].

Fyfe, Janet A. M. ;

Lavender, Caroline J. ;

Johnson, Paul D. R. ;

Globan, Maria ;

Sievers, Aina ;

Azuolas, Joseph ;

Stinear, Timothy P. .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (15) :4733-4740

[8] Mycobacterium ulcerans in mosquitoes captured during outbreak of buruli ulcer, Southeastern Australia [J].

Johnson, Paul D. R. ;

Azuolas, Joseph ;

Lavender, Caroline J. ;

Wishart, Elwyn ;

Stinear, Timothy P. ;

Hayman, John A. ;

Brown, Lynne ;

Jenkin, Grant A. ;

Fyfe, Janet A. M. .

EMERGING INFECTIOUS DISEASES, 2007, 13 (11) :1653-1660

[9] Real-Time Fluorescence Loop Mediated Isothermal Amplification for the Diagnosis of Malaria [J].

Lucchi, Naomi W. ;

Demas, Allison ;

Narayanan, Jothikumar ;

Sumari, Deborah ;

Kabanywanyi, Abdunoor ;

Kachur, S. Patrick ;

Barnwell, John W. ;

Udhayakumar, Venkatachalam .

PLOS ONE, 2010, 5 (10)

[10] Geographic Expansion of Buruli Ulcer Disease, Cameroon [J].

Marion, Estelle ;

Landier, Jordi ;

Boisier, Pascal ;

Marsollier, Laurent ;

Fontanet, Arnaud ;

Le Gall, Philippe ;

Aubry, Jacques ;

Djeunga, Noumen ;

Umboock, Alphonse ;

Eyangoh, Sara .

EMERGING INFECTIOUS DISEASES, 2011, 17 (03) :551-553

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