Cadmium modulates NADPH oxidase activity and expression in sunflower leaves

被引：53
作者：
Groppa, M. D. [1 ]
Ianuzzo, M. P. [1 ]
Rosales, E. P. [1 ]
Vazquez, S. C. [2 ]
Benavides, M. P. [1 ]
机构：
[1] Univ Buenos Aires, Fac Farm & Bioquim, Dept Quim Biol, Catedra Quim Biol Vegetal, RA-1113 Buenos Aires, DF, Argentina
[2] Univ Buenos Aires, Fac Farm & Bioquim, Catedra Microbiol Ind & Biotecnol, Dept Microbiol Inmunol & Biotecnol, RA-1113 Buenos Aires, DF, Argentina
来源：
BIOLOGIA PLANTARUM | 2012年 / 56卷 / 01期
关键词：
Helianthus annuus; reactive oxygen species; superoxide anion; APOPLASTIC OXIDATIVE BURST; OXYGEN SPECIES PRODUCTION; TOBACCO CELLS; CALCIUM-CHANNELS; NITRIC-OXIDE; STRESS; ACCUMULATION; GROWTH; PLANTS; INVOLVEMENT;
D O I：
10.1007/s10535-012-0036-z
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The production of reactive oxygen species (ROS) and the ways by which ROS are generated are very important facts related to heavy metal toxicity in plants. In this work, superoxide anion (O-2(center dot-)) generation diminished in cadmium treated sunflower (Helianthus annuus L.) leaf discs, and this reduction was time and Cd-concentration dependent. In line with these findings, we observed that NADPH-dependent oxidase activity was significantly inhibited by 0.1 and 0.5 mM Cd2+ treatments and the expression of the NADPH oxidase putative gene related to O-2(center dot-) synthesis in sunflower leaves was 83 % inhibited by 0.1 mM CdCl2 and almost completely depleted by 0.5 mM CdCl2.
引用
收藏
页码：167 / 171
页数：5
相关论文
共 37 条
[1]  
Asada K., 1994, Causes of photooxidative stress and amelioration of defense systems in plants., P77
[2]  
Benavides María P., 2005, Braz. J. Plant Physiol., V17, P21, DOI 10.1590/S1677-04202005000100001
[3]  
Bolwell GP, 1999, FREE RADICAL RES, V31, pS137
[4]   The apoplastic oxidative burst in response to biotic stress in plants: a three-component system [J].
Bolwell, GP ;
Bindschedler, LV ;
Blee, KA ;
Butt, VS ;
Davies, DR ;
Gardner, SL ;
Gerrish, C ;
Minibayeva, F .
JOURNAL OF EXPERIMENTAL BOTANY, 2002, 53 (372) :1367-1376
[5]   Cadmium-induced ultramorphological and physiological changes in leaves of two transgenic cotton cultivars and their wild relative [J].
Daud, M. K. ;
Variath, M. T. ;
Ali, Shafaqat ;
Najeeb, U. ;
Jamil, Muhammad ;
Hayat, Y. ;
Dawood, M. ;
Khan, Muhammad Imran ;
Zaffar, M. ;
Cheema, Sardar Alam ;
Tong, X. H. ;
Zhu, Shuijin .
JOURNAL OF HAZARDOUS MATERIALS, 2009, 168 (2-3) :614-625
[6]   The heme component of the neutrophil NADPH oxidase complex is a target for aryliodonium compounds [J].
Doussiere, J ;
Gaillard, J ;
Vignais, PV .
BIOCHEMISTRY, 1999, 38 (12) :3694-3703
[7]   Reactive oxygen species produced by NADPH oxidase regulate plant cell growth [J].
Foreman, J ;
Demidchik, V ;
Bothwell, JHF ;
Mylona, P ;
Miedema, H ;
Torres, MA ;
Linstead, P ;
Costa, S ;
Brownlee, C ;
Jones, JDG ;
Davies, JM ;
Dolan, L .
NATURE, 2003, 422 (6930) :442-446
[8]   Cadmium affects tobacco cells by a series of three waves of reactive oxygen species that contribute to cytotoxicity [J].
Garnier, Lionel ;
Simon-Plas, Francoise ;
Thuleau, Patrice ;
Agnel, Jean-Pierre ;
Blein, Jean-Pierre ;
Ranjeva, Raoul ;
Montillet, Jean-Luc .
PLANT CELL AND ENVIRONMENT, 2006, 29 (10) :1956-1969
[9]   Antioxidant metabolism of coffee cell suspension cultures in response to cadmium [J].
Gomes-Junior, Rui A. ;
Moldes, Carlos A. ;
Delite, Fabricio S. ;
Pompeu, Georgia B. ;
Gratao, Priscila L. ;
Mazzafera, Paulo ;
Lea, Peter J. ;
Azevedo, Ricardo A. .
CHEMOSPHERE, 2006, 65 (08) :1330-1337
[10]   Cadmium and mineral nutrient accumulation in potato plantlets grown under cadmium stress in two different experimental culture conditions [J].
Goncalves, Jamile Fabbrin ;
Antes, Fabiane Goldschmidt ;
Maldaner, Joseila ;
Pereira, Luciane Belmonte ;
Tabaldi, Luciane Almeri ;
Rauber, Renata ;
Rossato, Liana Veronica ;
Bisognin, Dilson Antonio ;
Dressler, Valderi Luiz ;
de Moraes Flores, Erico Marlon ;
Nicoloso, Fernando Teixeira .
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2009, 47 (09) :814-821
1234