Dynamic piezoelectric stimulation enhances osteogenic differentiation of human adipose stem cells

被引：154

作者：

Ribeiro, Clarisse ^{[1
,2
]}

Parssinen, Jenita ^{[3
,4
]}

Sencadas, Vitor ^{[1
,5
]}

Correia, Vitor ^{[1
,2
,6
]}

Miettinen, S. ^{[3
]}

Hytonen, Vesa P. ^{[3
,4
]}

Lanceros-Mendez, Senentxu ^{[1
,2
]}

机构：

[1] Univ Minho, Ctr Dept Phys, P-4710057 Braga, Portugal

[2] INL Int Iberian Nanotechnol Lab, P-4715330 Braga, Portugal

[3] Univ Tampere, BioMediTech, Tampere 33014, Finland

[4] Fimlab Labs Ltd, Tampere 33520, Finland

[5] Inst Politecn Cavado & Ave, P-4750810 Barcelos, Portugal

[6] Univ Minho, Algoritmi Res Ctr, P-4800058 Guimaraes, Portugal

来源：

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | 2015年 / 103卷 / 06期

基金：

芬兰科学院;

关键词：

electroactive polymer; stem cell; osteogenesis; dynamic conditions; SCAFFOLDS; TISSUE;

D O I：

10.1002/jbm.a.35368

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

This work reports on the influence of the substrate polarization of electroactive -poly(vinylidene fluoride) (-PVDF) on human adipose stem cells (hASCs) differentiation under static and dynamic conditions. hASCs were cultured on different -PVDF surfaces (non-poled and poled -) adsorbed with fibronectin and osteogenic differentiation was determined using a quantitative alkaline phosphatase assay. Poled - -PVDF samples promote higher osteogenic differentiation, which is even higher under dynamic conditions. It is thus demonstrated that electroactive membranes can provide the necessary electromechanical stimuli for the differentiation of specific cells and therefore will support the design of suitable tissue engineering strategies, such as bone tissue engineering. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 2172-2175, 2015.

引用

页码：2172 / 2175

页数：4

共 18 条

[1] Relevance of collagen piezoelectricity to "Wolff's Law": A critical review [J].

Ahn, Andrew C. ;

Grodzinsky, Alan J. .

MEDICAL ENGINEERING & PHYSICS, 2009, 31 (07) :733-741

[2] Cell differentiation by mechanical stress [J].

Altman, GH ;

Horan, RL ;

Martin, I ;

Farhadi, J ;

Stark, PRH ;

Volloch, V ;

Richmond, JC ;

Vunjak-Novakovic, G ;

Kaplan, DL .

FASEB JOURNAL, 2001, 15 (14) :270-+

[3] Enhanced osteoblastic differentiation of mesenchymal stem cells seeded in RGD-functionalized PLLA scaffolds and cultured in a flow perfusion bioreactor [J].

Alvarez-Barreto, Jose F. ;

Landy, Bonnie ;

VanGordon, Samuel ;

Place, Laura ;

DeAngelis, Paul L. ;

Sikavitsas, Vassilios I. .

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 2011, 5 (06) :464-475

[4] Functional tissue engineering: The role of biomechanics [J].

Butler, DL ;

Goldstein, SA ;

Guilak, F .

JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME, 2000, 122 (06) :570-575

[5] Differentiation of Stem Cells: Strategies for Modifying Surface Biomaterials [J].

Fu, Ru-Huei ;

Wang, Yu-Chi ;

Liu, Shih-Ping ;

Huang, Chin-Mao ;

Kang, Yun-Han ;

Tsai, Chang-Hai ;

Shyu, Woei-Cherng ;

Lin, Shinn-Zong .

CELL TRANSPLANTATION, 2011, 20 (01) :37-47

[6] ON THE PIEZOELECTRIC EFFECT OF BONE [J].

FUKADA, E ;

YASUDA, I .

JOURNAL OF THE PHYSICAL SOCIETY OF JAPAN, 1957, 12 (10) :1158-1162

[7] PIEZOELECTRIC AND RELATED PROPERTIES OF HYDRATED COLLAGEN [J].

FUKADA, E ;

UEDA, H ;

RINALDI, R .

BIOPHYSICAL JOURNAL, 1976, 16 (08) :911-918

[8] Geometric cues for directing the differentiation of mesenchymal stem cells [J].

Kilian, Kristopher A. ;

Bugarija, Branimir ;

Lahn, Bruce T. ;

Mrksich, Milan .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (11) :4872-4877

[9]

Lee YS, 2012, TISSUE ENG PT A, V18, P2063, DOI [10.1089/ten.tea.2011.0540, 10.1089/ten.TEA.2011.0540]

[10] Electroactive phases of poly(vinylidene fluoride): Determination, processing and applications [J].

Martins, P. ;

Lopes, A. C. ;

Lanceros-Mendez, S. .

PROGRESS IN POLYMER SCIENCE, 2014, 39 (04) :683-706

← 1 2 →