Early growth response gene 1 (EGR1) regulates heparanase gene transcription in tumor cells

被引:72
作者
de Mestre, AM
Rao, S
Hornby, JR
Soe-Htwe, T
Khachigian, LM
Hulett, MD [1 ]
机构
[1] Australian Natl Univ, John Curtin Sch Med Res, Div Immunol & Genet, Canc & Vasc Biol Grp, Acton, ACT 2601, Australia
[2] Univ New S Wales, Ctr Vasc Res, Sydney, NSW 2052, Australia
关键词
D O I
10.1074/jbc.M503414200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Heparanase is an endoglycosidase that degrades heparan sulfate chains of heparan sulfate proteoglycans, a key component of extracellular matrix and basement membranes. Studies using heparanase inhibitors and gene silencing have provided evidence to support an important role for heparanase in tumor metastasis and angiogenesis. The expression of heparanase is normally very tightly controlled, however, it is commonly deregulated in tumor cells, which express elevated heparanase activity that correlates with high levels of heparanase mRNA. We recently identified the transcription factor early growth response gene 1, EGR1, as a key regulator of inducible heparanase transcription in T cells. In this study using chromatin immunoprecipitation, we demonstrate for the first time that EGR1 binds to the heparanase gene promoter in vivo. The important question of the role of EGR1 in regulating heparanase transcription in tumor cells was then assessed. Studies were carried out in four epithelial tumor lines of different tissue origin. Functional dissection of the heparanase promoter identified a 280-bp region that was critical for transcription of the heparanase gene. Transactivation studies using an EGR1 expression vector co-transfected with a reporter construct containing the 280-bp region showed EGR1-activated heparanase promoter activity in a dose-dependent manner in prostate or breast adenocarcinoma and colon carcinoma cell lines. In contrast, overexpression of EGR1 resulted in a dose-dependent repression of promoter activity in melanoma cells. Using site-directed mutagenesis the 280-bp region was found to contain two functional EGR1 sites and electrophoretic mobility shift assays showed binding of EGR1 to both of these sites upon activation of tumor cells. Furthermore, the heparanase promoter region containing the EGR1 sites was also inducible in tumor cells and induction corresponded to HPSE expression levels. These studies show that EGR1 regulates heparanase transcription in tumor cells and importantly, can have a repressive or activating role depending on the tumor type.
引用
收藏
页码:35136 / 35147
页数:12
相关论文
共 69 条
  • [1] Site-directed mutagenesis, proteolytic cleavage, and activation of human proheparanase
    Abboud-Jarrous, G
    Rangini-Guetta, Z
    Aingorn, H
    Atzmon, R
    Elgavish, S
    Peretz, T
    Vlodavsky, I
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (14) : 13568 - 13575
  • [2] Impaired prostate tumorigenesis in Egr1-deficient mice
    Abdulkadir, SA
    Qu, ZC
    Garabedian, E
    Song, SK
    Peters, TJ
    Svaren, J
    Carbone, JM
    Naughton, CK
    Catalona, WJ
    Ackerman, JJH
    Gordon, JI
    Humphrey, PA
    Milbrandt, J
    [J]. NATURE MEDICINE, 2001, 7 (01) : 101 - 107
  • [3] Dominant-negative CREB inhibits heparanase functionality and melanoma cell invasion
    Aucoin, R
    Reiland, J
    Roy, M
    Marchetti, D
    [J]. JOURNAL OF CELLULAR BIOCHEMISTRY, 2004, 93 (02) : 215 - 223
  • [4] Antisense to the early growth response-1 gene (Egr-1) inhibits prostate tumor development in TRAMP mice
    Baron, V
    Duss, S
    Rhim, JH
    Mercola, D
    [J]. THERAPEUTIC OLIGONUCLEOTIDES: ANTISENSE, RNAI, TRIPLE-HELIX, GENE REPAIR, ENHANCER DECOYS, CPG AND DNA CHIPS, 2003, 1002 : 197 - 216
  • [5] Purification and characterization of the endoglycosidase heparanase 1 from human plantar stratum corneum:: a key enzyme in epidermal physiology?
    Bernard, D
    Méhul, B
    Delattre, C
    Simonetti, L
    Thomas-Collignon, A
    Schmidt, R
    [J]. JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2001, 117 (05) : 1266 - 1273
  • [6] Functions of cell surface heparan sulfate proteoglycans
    Bernfield, M
    Götte, M
    Park, PW
    Reizes, O
    Fitzgerald, ML
    Lincecum, J
    Zako, M
    [J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1999, 68 : 729 - 777
  • [7] Castellanos MD, 2002, EUR J IMMUNOL, V32, P3108
  • [8] Inflammatory cytokines and fatty acids regulate endothelial cell heparanase expression
    Chen, GP
    Wang, DY
    Vikramadithyan, R
    Yagyu, H
    Saxena, U
    Pillarisetti, S
    Goldberg, IJ
    [J]. BIOCHEMISTRY, 2004, 43 (17) : 4971 - 4977
  • [9] Histone dynamics on the interleukin-2 gene in response to T-Cell activation
    Chen, XX
    Wang, J
    Woltring, D
    Gerondakis, S
    Shannon, MF
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 2005, 25 (08) : 3209 - 3219
  • [10] Angiotensin II (ATII)-inducible platelet-derived growth factor A-chain gene expression is p42/44 extracellular signal-regulated kinase-1/2 and Egr-1-dependent and mediated via the ATII type 1 but not type 2 receptor - Induction by ATII antagonized by nitric oxide
    Day, FL
    Rafty, LA
    Chesterman, CN
    Khachigian, LM
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (34) : 23726 - 23733