Molecular characterization of yellow catfish (Pelteobagrus fulvidraco) IRF7 suggests involvement in innate immune response

被引：6
作者：
Liu, Xiaoxiao [1 ]
Lv, Xue [1 ]
Wu, Yeqing [1 ]
Song, Jingjing [1 ]
Wang, Xingguo [1 ]
Zhu, Rong [1 ]
机构：
[1] Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Hubei, Peoples R China
来源：
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2020年 / 109卷
基金：
中国国家自然科学基金;
关键词：
IRF7; Type I IFN; Pelteobagrus fulvidraco; Innate immune response; Antiviral effect; REGULATORY FACTOR 7; TOLL-LIKE RECEPTORS; FUNCTIONAL-CHARACTERIZATION; EXPRESSION ANALYSES; INTERFERON; CLONING; GENE; ACTIVATION; FISH; IDENTIFICATION;
D O I：
10.1016/j.dci.2020.103700
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
Interferon regulatory factor 7 (IRF7) serves as a critical mediator in the regulation of type I interferon (IFN) response to invading pathogens. Here, an ortholog of IRF7 was characterized in yellow catfish (Pelteobagrus fulvidraco). The full-length cDNA of PfIRF7 consisted of 1516 bp encoding a polypeptide of 425 amino acids. PfIRF7 protein comprised a typical IRF structural architecture, including a DNA binding domain (DBD), an IRF association domain (IAD) and a serine-rich domain (SRD). PfIRF7 was expressed predominantly in the immune-related tissues and transcriptionally upregulated by PolyI:C, LPS, and Edwardsiella ictaluri. Ectopic expression of PfIRF7 led to activation of fish type I IFN promoters and induction of IFN and Vigl, thereby conferring a strong antiviral effect against spring viremia of carp virus (SVCV). Overall, the present data suggest that PfIRF7 may play an essential role in type I IFN response of yellow catfish.
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页数：8
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