Nicotine Promotes Cell Migration Through Alpha7 Nicotinic Acetylcholine Receptor in Gastric Cancer Cells

被引：50
|
作者：
Lien, Yung-Chang [1 ,3 ]
Wang, Weu [1 ,3 ]
Kuo, Li-Jen [1 ,3 ,6 ]
Liu, Jun-Jen [4 ,5 ]
Wei, Po-Li [1 ,2 ,3 ,7 ]
Ho, Yuan-Soon [4 ,5 ,7 ]
Ting, Wen-Chien [8 ]
Wu, Chih-Hsiung [1 ,3 ,6 ,9 ]
Chang, Yu-Jia [1 ,2 ,3 ,6 ,7 ]
机构：
[1] Taipei Med Univ Hosp, Dept Surg, Div Gen Surg, Taipei, Taiwan
[2] Taipei Med Univ, Taipei Med Univ Hosp, Ctr Canc, Taipei, Taiwan
[3] Taipei Med Univ, Coll Med, Sch Med, Dept Surg, Taipei, Taiwan
[4] Taipei Med Univ, Coll Med Sci & Technol, Sch Med, Lab Sci & Biotechnol, Taipei, Taiwan
[5] Taipei Med Univ, Coll Med, Grad Inst Med Sci, Taipei, Taiwan
[6] Taipei Med Univ, Coll Med, Grad Inst Clin Med, Taipei, Taiwan
[7] Taipei Med Univ, Ctr Excellence Canc Res, Taipei, Taiwan
[8] Chung Shan Med Univ Hosp, Dept Surg, Div Colorectal Surg, Taichung, Taiwan
[9] Taipei Med Univ, Shuang Ho Hosp, Dept Surg, Div Gen Surg, Taipei, Taiwan
来源：
ANNALS OF SURGICAL ONCOLOGY | 2011年 / 18卷 / 09期
关键词：
EPITHELIAL-MESENCHYMAL TRANSITION; PROTEIN-KINASE-C; TUMOR-GROWTH; PROLIFERATION; ANGIOGENESIS; INVASION; DIFFUSE; METHYLATION; EXPRESSION; SNAIL;
D O I：
10.1245/s10434-011-1598-2
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The objective was to study the mechanism of nicotine-enhanced migration of gastric cancer cells. Long-term cigarette smoking increases the risk of gastric cancer mortality. Tobacco-specific mitogen, nicotine, was reported to correlate with cancer progression on gastric cancer. Since metastasis is the major cause of cancer death, the influence of nicotine on the migration of gastric cancer cells remains to be determined. The influence of nicotine on migration of gastric cancer cells was evaluated by transwell assay and wound-healing migration assay. Receptor-mediated migration was studied by both inhibitor and small interfering RNA. Alpha7 nicotinic acetylcholine receptor, alpha7-nAChR, was identified in gastric cancer cell lines, AGS cells. Nicotine enhanced AGS cell migration in transwell assay and wound-healing migration assay in a dose-dependent manner. We used inhibitor and siRNA to demonstrate that alpha7-nAChR mediated nicotine-enhanced gastric cancer cell migration through downregulation E-cadherin and upregulation ZEB-1 and snail. Tobacco-specific mitogen, nicotine, enhanced gastric cancer metastasis through alpha7-nAChR and suppression of E-cadherin level-one of the hallmarks of epithelial to mesenchymal transition. Therefore, patients with gastric cancer should avoid smoking.
引用
收藏
页码：2671 / 2679
页数：9
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