A different gut microbial community between larvae and adults of a wild bumblebee nest (Bombus pascuorum)

被引：26

作者：

Parmentier, Anneleen ^{[1
]}

Meeus, Ivan ^{[1
]}

Van Nieuwerburgh, Filip ^{[2
]}

Deforce, Dieter ^{[2
]}

Vandamme, Peter ^{[3
]}

Smagghe, Guy ^{[1
]}

机构：

[1] Univ Ghent, Fac Biosci Engn, Dept Crop Protect, Lab Agrozool, Coupure Links 653, B-9000 Ghent, Belgium

[2] Univ Ghent, Fac Pharmaceut Sci, Lab Pharmaceut Biotechnol, Ghent, Belgium

[3] Univ Ghent, Dept Biochem & Microbiol, Lab Microbiol, Fac Sci, Ghent, Belgium

来源：

INSECT SCIENCE | 2018年 / 25卷 / 01期

关键词：

bumblebee; gut; Illumina sequencing; larvae; microbiota; nest; sample size; HONEY-BEE; GEN; NOV; DIVERSITY; BACTERIA; TERRESTRIS; SEQUENCES;

D O I：

10.1111/1744-7917.12381

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Although the gut microbial communities in adult bumblebees and their associated functionalities are widely studied, descriptive data on the larval gut microbiota are still limited. The gut microbiota of a fully sampled wild Bombus pascuorum nest has been characterized, using the multiplexed Illumina MiSeq 16S ribosomal RNA amplicon sequencing technique. The nesters and foragers inhabiting the same nest showed the typical core bacterial sequences and only marginal differences in their characterized gut microbiota. The gut microbial communities within the adult and larval specimens differed strongly, as the typical core gut bacteria in the adult bumblebees are absent in the larval bumblebees. The bacterial communities within the larval gut are dominated by bacterial phylotypes of Enterobacteriaceae and Lactobacillaceae, supplemented with genera belonging to Corynebacteriales and Bacillales. The function of this larval gut microbiota, being different from the adult, remains to be determined.

引用

页码：66 / 74

页数：9

共 33 条

[1] Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample [J].