Equine viral arteritis in a newborn foal:: Parallel detection of the virus by immunohistochemistry, polymerase chain reaction and virus isolation

被引:14
作者
Szeredi, L [1 ]
Hornyák, A [1 ]
Dénes, B [1 ]
Rusvai, M [1 ]
机构
[1] Cent Vet Inst, Budapest, Hungary
来源
JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH | 2003年 / 50卷 / 06期
关键词
INFECTION; PROTEIN;
D O I
10.1046/j.1439-0450.2003.00684.x
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
A 4-days-old foal died after a short course of respiratory syndrome and fever. Large areas of the alveoli, bronchioles and bronchi were partly or completely filled by hyaline membranes. Pronounced oedema and mild interstitial pneumonia were present and, in the small muscular arteries, fibrinoid necrosis and vasculitis or perivasculitis could be seen. Vasculitis was found in several other organs, and it was most severe in the thymus. The virus was detected in the lung, kidney and spleen using virus isolation and in the lung and spleen using polymerase chain reaction. The virus was also detected in several organs and cell types using both N protein-specific monoclonal antibody and horseradish peroxidase-labelled equine arteritis virus-specific equine IgG.
引用
收藏
页码:270 / 274
页数:5
相关论文
共 18 条
[1]  
BALASURIYA UBR, 1999, P 8 INT C EQ INF DIS
[2]   COMPARISON OF M-GENE AND N-GENE SEQUENCES DISTINGUISHES VARIATION AMONGST EQUINE ARTERITIS VIRUS ISOLATES [J].
CHIRNSIDE, ED ;
WEARING, CM ;
BINNS, MM ;
MUMFORD, JA .
JOURNAL OF GENERAL VIROLOGY, 1994, 75 :1491-1497
[3]  
CRAWFORD TB, 1973, P INT C EQ INF DIS, V3, P282
[4]   Equine viral arteritis [J].
Del Piero, F .
VETERINARY PATHOLOGY, 2000, 37 (04) :287-296
[5]  
DelPiero F, 1997, EQUINE VET J, V29, P178, DOI 10.1111/j.2042-3306.1997.tb01666.x
[6]  
ESTES PC, 1970, AM J PATHOL, V58, P235
[7]  
GOLNIK W, 1981, SCHWEIZ ARCH TIERH, V123, P523
[8]  
LITTLE TV, 1995, P 7 INT C EQ INF DIS, P27
[9]   Immunoperoxidase histochemistry as a diagnostic tool for detection of equine arteritis virus antigen in formalin fixed tissues [J].
Lopez, JW ;
DelPiero, F ;
Glaser, A ;
Finazzi, M .
EQUINE VETERINARY JOURNAL, 1996, 28 (01) :77-79
[10]   Fatal experimental equine arteritis virus infection of a pregnant mare: Immunohistochemical staining of viral antigens [J].
MacLachlan, NJ ;
Balasuriya, UBR ;
Rossitto, PV ;
Hullinger, PA ;
Patton, JF ;
Wilson, WD .
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 1996, 8 (03) :367-374