Long term recovery of median nerve repair using laser-activated chitosan adhesive films

被引:23
作者
Barton, Matthew J. [1 ]
Morley, John W. [1 ,2 ]
Stoodley, Marcus A. [3 ]
Shaikh, Sumaiya [1 ]
Mahns, David A. [1 ,2 ]
Lauto, Antonio [1 ,2 ]
机构
[1] Univ Western Sydney, Sch Med, Penrith, NSW 2751, Australia
[2] Univ Western Sydney, MARCS Inst, Bioelect & Neurosci BENS Res Grp, Penrith, NSW 2751, Australia
[3] Macquarie Univ, Australian Sch Adv Med, Sydney, NSW 2109, Australia
关键词
biomaterial; chitosan; laser; peripheral-nerve-repair; photochemical-tissue-bonding and rose-bengal; PROTEIN BAND TECHNIQUE; RAT SCIATIC-NERVE; IN-VITRO; PERIPHERAL-NERVES; SCHWANN-CELLS; FIBRIN GLUE; ROSE-BENGAL; TISSUE; REGENERATION; SUTURE;
D O I
10.1002/jbio.201300129
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Sutures remain the standard peripheral nerve repair technique, whether applied directly or indirectly to nerve tissue. Unfortunately, significant postoperative complications can result, such as inflammation, neuroma formation and foreign body reactions. Photochemical-tissue-bonding (PTB) using rose Bengal (RB) integrated into a chitosan bioadhesive is an alternative nerve repair device that removes the need for sutures. Rats were arranged into three groups: RB-chitosan adhesives-repair, end-to-end epineural suture-repair (surgical standard) and sham laser-irradiated control. Groups were compared through histological assessment, electrophysiological recordings and grip motor strength. RB-chitosan adhesive repaired nerves displayed comparable results when compared to the standard suture-repair based on histological and electrophysiological findings. Functionally, RB-chitosan adhesive was associated with a quicker and more pronounced recovery of grip force when compared to the suture-repair. [GRAPHICS] Longitudinal sections of nerve anastomosis after 4 weeks (top) and 12 weeks (bottom); the nerves were repaired using the laser-adhesive technique (scale bar 50 mu m).
引用
收藏
页码:196 / 207
页数:12
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