Molecular Phylogenetic Analysis of Phyllanthus Species in Thailand and the Application of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism for Phyllanthus amarus Identification

被引：9
|
作者：
Manissorn, Juthatip [2 ,3 ]
Sukrong, Suchada [2 ,3 ]
Ruangrungsi, Nijsiri [3 ]
Mizukami, Hajime [1 ]
机构：
[1] Nagoya City Univ, Grad Sch Pharmaceut Sci, Mizuho Ku, Nagoya, Aichi 4678603, Japan
[2] Chulalongkorn Univ, CU Drugs & Hlth Prod Innovat & Promot Ctr, Fac Pharmaceut Sci, Bangkok 10330, Thailand
[3] Chulalongkorn Univ, Dept Pharmacognosy & Pharmaceut Bot, Fac Pharmaceut Sci, Bangkok 10330, Thailand
来源：
BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2010年 / 33卷 / 10期
关键词：
Phyllanthus; molecular phylogenetic analysis; internal transcribed spacer; DNA authentication; EUPHORBIACEAE; BIOASSAYS; SEQUENCE; CONSTITUENTS; COMPILATION; MARKERS; USAGE;
D O I：
10.1248/bpb.33.1723
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
The genus Phyllanthus (Phyllanthaceae) is distributed in tropical and subtropical regions, and its members are widely used as medicinal plants in many countries. We analyzed the nucleotide sequences of the internal transcribed spacers of ribosomal DNA of 56 plant samples covering 23 Phyllanthus species collected from various habitats in Thailand. Based on the sequence alignment, we constructed phylogenetic trees of all Phyllanthus species distributed in Thailand. Furthermore, a simple protocol to discriminate three important medicinal Phyllanthus species, P amarus, P debilis, and P urinaria, was developed using a Polymerase Chain Reaction-Restriction Fragment Length Polymorphism method and successfully applied to the crude drug samples obtained in Thai markets.
引用
收藏
页码：1723 / 1727
页数：5
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