Sago starch as a biomass source: Raw sago starch hydrolysis by commercial enzymes

被引：40

作者：

Wang, WJ ^{[1
]}

Powell, AD ^{[1
]}

Oates, CG ^{[1
]}

机构：

[1] NATL UNIV SINGAPORE, FAC MED, DEPT BOT, SINGAPORE 117548, SINGAPORE

来源：

BIORESOURCE TECHNOLOGY | 1996年 / 55卷 / 01期

关键词：

amylase; glucoamylase; sage starch; raw starch hydrolysis; starch oligosaccharide profiles;

D O I：

10.1016/0960-8524(95)00132-8

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Raw sage starch and sage starch pretreated by heating at 60 degrees C for 2 hours in sodium acetate buffer (pH 3.5) were hydrolysed using commercial glucoamylase-AMG (EC 3.2.1.3), alpha-amylases-BAN, Fungamyl and Termamyl (EC 3.2.1.1), debranching amylase-Promozyme (EC 3.2.1.41), and their mixtures in sodium acetate buffer pH 5.0 at 35 degrees C. Raw sage starch was a poor substrate for enzyme action compared to corn and tapioca starches tested under the same conditions, although pretreating the starch increased the extent and rate of hydrolysis. A strong synergism between glucoamylase and alpha-amylase on the hydrolysis of both untreated and pretreated sage starch was observed The hydrolysis products were characterized by high-performance size-exclusion chromatography (HPSEC). The total carbohydrate concentration of hydrolysed sage starch decreased but the amylose and amylopectin ratios in the residues remained the same. (C) 1996 Elsevier Science Ltd.

引用

页码：55 / 61

页数：7

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