A simple screening procedure for heterotrophic nitrifying bacteria with oxygen-tolerant denitrification activity

被引:26
|
作者
Matsuzaka, E [1 ]
Nomura, N [1 ]
Nakajima-Kambe, T [1 ]
Okada, N [1 ]
Nakaharai, T [1 ]
机构
[1] Univ Tsukuba, Inst Appl Biochem, Tsukuba, Ibaraki 3058582, Japan
基金
日本科学技术振兴机构;
关键词
nitrification; heterotrophic nitrifying bacteria; denitrification;
D O I
10.1263/jbb.95.409
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Various naturally occurring strains of heterotrophic nitrifying bacteria were isolated by enrichment culture using acetamide as the C and N source, and 21 strains were identified as heterotrophic nitrifiers. Using a new simple procedure, these 21 strains were also investigated for the ability to carry out denitrification in the presence of oxygen. Several of the nitrifying strains were found to exhibit a distinct activity that allows for denitrification via nitrite (NO2-) in the presence of oxygen, indicating that they have an oxygen-tolerant denitrification system. A wide variety of bacteria possessing both nitrification and denitrification capabilities in the presence of oxygen were isolated and partially characterized by using the simple screening combinatorial procedure described in this paper.
引用
收藏
页码:409 / 411
页数:3
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