Sau-PCR, a novel amplification technique for genetic fingerprinting of microorganisms

被引：22

作者：

Corich, V ^{[1
]}

Mattiazzi, A ^{[1
]}

Soldati, E ^{[1
]}

Carraro, A ^{[1
]}

Giacomini, A ^{[1
]}

机构：

[1] Univ Padua, Dipartimento Biotecnol Agr, I-35020 Legnaro, Padova, Italy

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2005年 / 71卷 / 10期

关键词：

D O I：

10.1128/AEM.71.10.6401-6406.2005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The proposed technique is based on the digestion of genomic DNA with the restriction endonuclease Sau3AI and subsequent amplification with primers whose core sequence is based on the Sau3AI recognition site. The method was tested on strains of lactic acid bacteria but could be proposed for virtually any culturable organism from which DNA can be extracted.

引用

页码：6401 / 6406

页数：6

共 24 条

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