Whole-genome sequencing of Staphylococcus epidermidis bloodstream isolates from a prospective clinical trial reveals that complicated bacteraemia is caused by a limited number of closely related sequence types

被引：13

作者：

Shelburne, S. A. ^{[1
,2
]}

Dib, R. W. ^{[1
]}

Endres, B. T. ^{[3
]}

Reitzel, R. ^{[1
]}

Li, X. ^{[4
]}

Kalia, A. ^{[4
]}

Sahasrabhojane, P. ^{[1
]}

Chaftari, A-M ^{[1
]}

Hachem, R. ^{[1
]}

Vargas-Cruz, N. S. ^{[1
]}

Jiang, Y. ^{[1
]}

Garey, K. ^{[3
]}

Fowler, V. G. ^{[5
]}

Holland, T. L. ^{[5
]}

Gu, J. ^{[6
]}

Miller, W. ^{[7
]}

Sakurai, A. ^{[7
]}

Arias, C. A. ^{[7
,8
,9
,10
]}

Aitken, S. L. ^{[11
]}

Greenberg, D. E. ^{[12
,13
]}

Kim, J. ^{[14
]}

Flores, A. R. ^{[15
]}

Raad, I ^{[1
]}

机构：

[1] MD Anderson Canc Ctr, Dept Infect Dis, Houston, TX 77030 USA

[2] MD Anderson Canc Ctr, Dept Genom Med, Houston, TX 77030 USA

[3] Univ Houston, Coll Pharm, Dept Pharm Practice & Translat Res, Houston, TX 77030 USA

[4] MD Anderson Canc Ctr, Grad Program Diagnost Genet, Houston, TX 77030 USA

[5] Duke Univ, Med Ctr, Div Infect Dis, Durham, NC USA

[6] Duke Clin Res Inst, Durham, NC USA

[7] Univ Texas Houston, McGovern Med Sch Houston, Div Infect Dis, Houston, TX USA

[8] Univ Texas Houston, McGovern Med Sch Houston, Ctr Antimicrobial Resistance & Microbial Genom, Houston, TX USA

[9] Univ El Bosque, Mol Genet & Antimicrobial Resistance Unit, Bogota, Colombia

[10] Univ El Bosque, Int Ctr Microbial Genom, Bogota, Colombia

[11] MD Anderson Canc Ctr, Div Pharm, Houston, TX 77030 USA

[12] UT Southwestern, Dept Internal Med, Dallas, TX USA

[13] UT Southwestern, Dept Microbiol, Dallas, TX USA

[14] UT Southwestern Med Ctr, Dept Bioinformat, Dallas, TX USA

[15] Univ Texas Houston, Hlth Sci Ctr, Dept Pediat, McGovern Med Sch, Houston, TX 77225 USA

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2020年 / 26卷 / 05期

基金：

美国国家卫生研究院;

关键词：

Staphylococcus epidermidis; Whole-genome sequencing; Genotype:phenotype correlation; Antimicrobial resistance; Phylogenomics; INFECTIONS; CARE; COLONIZATION; EPIDEMIOLOGY; VANCOMYCIN; RESISTANCE; AUREUS;

D O I：

10.1016/j.cmi.2019.10.008

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Objectives: The significance of isolating Staphylococus epidermidis from a blood culture is highly heterogeneous, ranging from contamination to an indication of a serious infection. Herein we sought to determine whether there is a relationship between S. epidermidis genotype and clinical severity of bacteraemia. Methods: S. epidermidis bacteraemias from a prospective, multicentre trial at 15 centres in the United States and one in Spain were classified as simple (including possible contamination), uncomplicated, and complicated. Whole-genome sequencing (WGS) was performed on 161 S. epidermidis isolates, and clinical outcomes were correlated with genotypic information. Results: A total of 49 S. epidermidis sequence types (STs) were identified. Although strains of all 49 STs were isolated from patients with either simple or uncomplicated infection, all strains causing complicated infections were derived from five STs: ST2, ST5, ST7, ST16, and ST32. ST2 and ST5 isolates were significantly more likely to cause uncomplicated and complicated bloodstream infections compared to simple bacteraemia (odds ratio 2.0, 95%CI 1.1-3.9, p 0.04). By multivariate regression analysis, having an ST2 or ST5 S. epidermidis bacteraemia was an independent predictor of complicated bloodstream infection (odds ratio 3.7, 95%CI 1.2-11.0, p 0.02). ST2/ST5 strains carried larger numbers of antimicrobial resistance determinants compared to non-ST2/ST5 isolates (6.34 +/- 1.5 versus 4.4 +/- 2.5, p < 0.001). Conclusion: S. epidermidis bacteraemia was caused by a genetically heterogeneous group of organisms, but only a limited number of STs-particularly multidrug-resistant ST2 and ST5 strains-caused complicated infections. (C) 2019 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

引用

页码：646.e1 / 646.e8

页数：8

共 2 条

[1] Whole-Genome Sequencing of Staphylococcus aureus and Staphylococcus haemolyticus Clinical Isolates from Egypt
Montelongo, Cesar
Mores, Carine R.
Putonti, Catherine
Wolfe, Alan J.
Abouelfetouh, Alaa
MICROBIOLOGY SPECTRUM, 2022, 10 (04):
[2] Whole Genome Sequencing of Methicillin-Resistant Staphylococcus epidermidis Clinical Isolates Reveals Variable Composite SCCmec ACME among Different STs in a Tertiary Care Hospital in Oman
Al-Jabri, Zaaima
AL-Shabibi, Zahra
AL-Bimani, Atika
AL-Hinai, Amal
AL-Shabibi, Ammar
Rizvi, Meher
MICROORGANISMS, 2021, 9 (09)

← 1 →