Non-invasive sample collection for respiratory virus testing by multiplex PCR

被引:38
作者
Blaschke, Anne J. [1 ]
Allison, Mandy A.
Meyers, Lindsay [2 ]
Rogatcheva, Margarita [2 ]
Heyrend, Caroline [2 ]
Mallin, Brittany [3 ]
Carter, Marjorie [3 ]
LaFleur, Bonnie [4 ]
Barney, Trenda [5 ]
Poritz, Mark A. [2 ]
Daly, Judy A. [5 ,6 ]
Byington, Carrie L.
机构
[1] Univ Utah, Dept Pediat, Div Pediat Infect Dis, Salt Lake City, UT 84108 USA
[2] Idaho Technol Inc, Salt Lake City, UT USA
[3] Univ Utah, Dept Internal Med, Salt Lake City, UT 84108 USA
[4] Univ Arizona, Coll Publ Hlth, Tucson, AZ USA
[5] Primary Childrens Med Ctr, Microbiol Lab, Salt Lake City, UT 84103 USA
[6] Univ Utah, Dept Pathol, Salt Lake City, UT 84108 USA
关键词
Respiratory virus; Viral diagnostics; Molecular diagnostics; Pediatrics; Upper respiratory infection; Polymerase chain reaction; PEDIATRIC EMERGENCY-DEPARTMENT; POLYMERASE-CHAIN-REACTION; RAPID DIAGNOSIS; FLOCKED SWABS; NASOPHARYNGEAL ASPIRATE; VIRAL-INFECTIONS; CONTROLLED-TRIAL; INFLUENZA-B; NASAL SWAB; RT-PCR;
D O I
10.1016/j.jcv.2011.07.015
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Background: Identifying respiratory pathogens within populations is difficult because invasive sample collection, such as with nasopharyngeal aspirate (NPA), is generally required. PCR technology could allow for non-invasive sampling methods. Objective: Evaluate the utility of non-invasive sample collection using anterior nare swabs and facial tissues for respiratory virus detection by multiplex PCR. Study design: Children aged 1 month-17 years evaluated in a pediatric emergency department for respiratory symptoms had a swab, facial tissue, and NPA sample collected. All samples were tested for respiratory viruses by multiplex PCR. Viral detection rates were calculated for each collection method. Sensitivity and specificity of swabs and facial tissues were calculated using NPA as the gold standard. Results: 285 samples from 95 children were evaluated (92 swab-NPA pairs, 91 facial tissue-NPA pairs). 91% of NPA, 82% of swab, and 77% of tissue samples were positive for >= 1 virus. Respiratory syncytial virus (RSV) and human rhinovirus (HRV) were most common. Overall, swabs were positive for 74% of virus infections, and facial tissues were positive for 58%. Sensitivity ranged from 17 to 94% for swabs and 33 to 84% for tissues. Sensitivity was highest for RSV (94% swabs and 84% tissues). Specificity was >= 95% for all viruses except HRV for both collection methods. Conclusions: Sensitivity of anterior nare swabs and facial tissues in the detection of respiratory viruses by multiplex PCR varied by virus type. Given its simplicity and specificity, non-invasive sampling for PCR testing may be useful for conducting epidemiologic or surveillance studies in settings where invasive testing is impractical or not feasible. (C) 2011 Elsevier B. V. All rights reserved.
引用
收藏
页码:210 / 214
页数:5
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