A polymer lab-on-a-chip for magnetic immunoassay with on-chip sampling and detection capabilities

被引:103
作者
Do, Jaephil [1 ,2 ]
Ahn, Chong H. [1 ,2 ]
机构
[1] Univ Cincinnati, Dept Elect & Comp Engn, Ctr BioMEMS & Nanobiosci, Microsyst & BioMEMS Lab, Cincinnati, OH 45221 USA
[2] Univ Cincinnati, Dept Comp Sci, Ctr BioMEMS & Nanobiosci, Microsyst & BioMEMS Lab, Cincinnati, OH 45221 USA
关键词
D O I
10.1039/b715569g
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This paper presents a new polymer lab-on-a-chip for magnetic bead-based immunoassay with fully on-chip sampling and detection capabilities, which provides a smart platform of magnetic immunoassay-based lab-on-a-chip for point-of-care testing (POCT) toward biochemical hazardous agent detection, food inspection or clinical diagnostics. In this new approach, the polymer lab-on-a-chip for magnetic bead-based immunoassay consists of a magnetic bead-based separator, an interdigitated array (IDA) micro electrode, and a microfluidic system, which are fully incorporated into a lab-on-a-chip on cyclic olefin copolymer (COC). Since the polymer lab-on-a-chip was realized using low cost, high throughput polymer microfabrication techniques such as micro injection molding and hot embossing method, a disposable polymer lab-on-a-chip for the magnetic bead-based immunoassay can be successfully realized in a disposable platform. With this newly developed polymer lab-on-a-chip, an enzyme-labelled electrochemical immunoassay (ECIA) was performed using magnetic beads as the mobile solid support, and the final enzyme product produced from the ECIA was measured using chronoamperometry. A sampling and detection of as low as 16.4 ng mL(-1) of mouse IgG has been successfully performed in 35 min for the entire procedure.
引用
收藏
页码:542 / 549
页数:8
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