IgG deposition and activation of the classical complement pathway involvement in the activation of human granulocytes by decellularized porcine heart valve tissue

被引：36

作者：

Bastian, Fabienne ^{[1
,3
]}

Stelzmueller, Marie-Elisabeth ^{[1
]}

Kratochwill, Klaus ^{[2
]}

Kasimir, Marie-Theres ^{[1
]}

Simon, Paul ^{[1
]}

Weigel, Guenter ^{[1
,3
]}

机构：

[1] Med Univ Vienna, Dept Cardiothorac Surg, A-1090 Vienna, Austria

[2] Med Univ Vienna, Dept Pediat & Adolescent Med, RCU Pediat Biochem & Analyt, A-1090 Vienna, Austria

[3] Ludwig Boltzmann Cluster Cardiovasc Res, Vienna, Austria

来源：

BIOMATERIALS | 2008年 / 29卷 / 12期

关键词：

complement; extracellular matrix; inflammation; leukocytes; heart valve;

D O I：

10.1016/j.biomaterials.2008.01.005

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Decellularization treatment of heart valves has been thought to eliminate tissue immunogenicity. Early failure of tissue-engineered xenogeneic heart valves was seen in children and has been a major drawback in this promising field of research. This study was designed to characterize the effects of acellular porcine heart valve tissue on immune activation in vitro. Incubation of decellularized porcine tissue with human plasma led to adsorption of IgG, activation of the classical complement pathway and adhesion of activated polymorphonuclear leukocytes (PMN). This inflammatory response was strongly inhibited by proteins extracted from native porcine tissue which might indicate that inhibitors of PMN activation present in the extracellular matrix (ECM) are lost during the decellularization process. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：1824 / 1832

页数：9

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