Hypotonic stimuli enhance proton-gated currents of acid-sensing ion channel-1b

被引:4
作者
Ugawa, Shinya [1 ]
Ishida, Yusuke [1 ]
Ueda, Takashi [1 ]
Yu, Yong [1 ]
Shimada, Shoichi [1 ]
机构
[1] Nagoya City Univ, Grad Sch Med Sci, Dept Neurobiol & Anat, Mizuho Ku, Nagoya, Aichi 4678601, Japan
关键词
acid-sensing ion channel (ASIC); mechanoreceptor; oocyte electrophysiology; proton; hypotonicity; amiloride; hair cell;
D O I
10.1016/j.bbrc.2007.12.096
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Acid-sensing ion channels (ASICs) are strong candidates for mammalian mechanoreceptors. We investigated whether mouse acidsensing ion channel-1b (ASIC1b) is sensitive to mechanical stimuli using oocyte electrophysiology, because ASIC1b is located in the mechanosensory stereocilia of cochlear hair cells. Hypotonic stimuli that induced membrane stretch of oocytes evoked no significant current in ASIC1b-expressing oocytes at pH 7.5. However, acid (pH 4.0 or 5.0)-evoked currents in the oocytes were substantially enhanced by the hypotonicity, showing mechanosensitivity of ASIC1b and possible mechanogating of the channel in the presence of other components. Interestingly, the ASIC1b channel was permeable to K+ (a principal charge carrier for cochlear sensory transduction) and the affinity of the channel for antiloride (IC50 (inhibition constant) = approximately 48.3 mu M) was quite similar to that described for the mouse hair cell mechanotransducer current. Taken together, these data raise the possibility that ASIC1b participates in cochlear mechanoelectrical transduction. (C) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码:530 / 534
页数:5
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