Broad-band attenuated total reflection spectroscopy of a hydrated protein film on a single mode planar waveguide

被引:42
作者
Mendes, SB [1 ]
Li, LF [1 ]
Burke, JJ [1 ]
Lee, JE [1 ]
Dunphy, DR [1 ]
Saavedra, SS [1 ]
机构
[1] UNIV ARIZONA,DEPT CHEM,TUCSON,AZ 85721
关键词
D O I
10.1021/la9601162
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The development of a multichannel spectrometer based on single mode planar integrated optical waveguide and capable of measuring broad-band visible absorbance spectra of a weakly absorbing molecular blm at a solid-liquid interface is reported. The system was successfully tested by measuring spectra, over a 100 nn bandwidth, of a hydrated film of cytochrome c adsorbed to a glass waveguide surface. The approach should prove useful in spectral characterization of structure and function in thin film molecular assemblies at condensed phase interfaces.
引用
收藏
页码:3374 / 3376
页数:3
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