Broad-band attenuated total reflection spectroscopy of a hydrated protein film on a single mode planar waveguide

被引：42

作者：

Mendes, SB ^{[1
]}

Li, LF ^{[1
]}

Burke, JJ ^{[1
]}

Lee, JE ^{[1
]}

Dunphy, DR ^{[1
]}

Saavedra, SS ^{[1
]}

机构：

[1] UNIV ARIZONA,DEPT CHEM,TUCSON,AZ 85721

来源：

LANGMUIR | 1996年 / 12卷 / 14期

关键词：

D O I：

10.1021/la9601162

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The development of a multichannel spectrometer based on single mode planar integrated optical waveguide and capable of measuring broad-band visible absorbance spectra of a weakly absorbing molecular blm at a solid-liquid interface is reported. The system was successfully tested by measuring spectra, over a 100 nn bandwidth, of a hydrated film of cytochrome c adsorbed to a glass waveguide surface. The approach should prove useful in spectral characterization of structure and function in thin film molecular assemblies at condensed phase interfaces.

引用

页码：3374 / 3376

页数：3

共 40 条

[1] USE OF SELF-ASSEMBLED MONOLAYERS TO COVALENTLY TETHER PROTEIN MONOLAYERS TO THE SURFACE OF SOLID SUBSTRATES [J].