Characterization of the rice NLA family reveals a key role for OsNLA1 in phosphate homeostasis

Background: Phosphate (Pi), an essential mineral nutrient for plant development and reproduction, is one of the main components of fertilizers in modern agriculture. Previous research demonstrated that AtNLA1 mediates ubiquitination of Pi transporters in the plasma membrane and triggers their endocytosis and degradation in Arabidopsis. In this study, we researched the function of NLA homologous proteins in Pi homeostasis in rice. Findings: Two OsNLA homologs from rice (Oryza sativa L.) were identified by bioinformatics and phylogenetic analysis and designated OsNLA1 and OsNLA2. The OsNLA1 clustered with Arabidopsis AtNLA1, was expressed higher than OsNLA2 and was transcriptionally repressed under Pi-deficient condition. Loss-of-function of OsNLA1 caused P overaccumulation and growth inhibitions in both root and shoot under Pi-sufficient condition. Furthermore, mutation of OsNLA1 affected expression of Pi tranporters and root hair development under Pi-sufficient and/or Pi-deficient conditions. Conclusions: OsNLA1 plays a key role in maintaining phosphate homeostasis in rice.