Validation of a UV-spectrophotometric analytical method for determination of LPSF/AC04 from inclusion complex and liposomes

被引：5

作者：

Ferraz, Rafaela Siqueira ^{[1
]}

Moura Mendonca, Elisangela Afonso ^{[2
]}

Avelino Silva, Jessica Priscila ^{[1
]}

Ferro Cavalcanti, Isabella Macario ^{[1
,3
]}

Britto Lira-Nogueira, Mariane Cajuba ^{[1
,3
]}

Galdino, Suely Lins ^{[4
]}

Pitta, Ivan Rocha ^{[4
]}

Alves Lima, Maria do Carmo ^{[4
]}

Santos-Magalhaes, Nereide Stela ^{[1
]}

机构：

[1] Univ Fed Pernambuco, Lab Imunopatol Keizo Asami, Recife, PE, Brazil

[2] Univ Estadual Paraiba, Lab Sintese & Vetorizacao Mol, Joao Pessoa, Paraiba, Brazil

[3] CAV, Recife, PE, Brazil

[4] Univ Fed Pernambuco, Lab Planejamento & Sintese Farmacos, Dept Antibiot, Recife, PE, Brazil

来源：

BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES | 2015年 / 51卷 / 01期

关键词：

UV Spectrophotometry/quantitative analysis/method validation; Acridine derivatives/determination; Inclusion complex; Liposomes; ACRIDINE; CYCLODEXTRIN; SOLUBILITY; AGENTS;

D O I：

10.1590/S1984-82502015000100018

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The aim of this study was to develop and validate a UV spectrophotometric method for determination of LPSF/AC04 from inclusion complex and encapsulated into liposomes. The validation parameters were determined according to the International Conference on Harmonisation (ICH) and National Health Surveillance Agency (ANVISA) guidelines. LPSF/AC04 was determined at 250 nm in methanol by a UV spectrophotometric method, exhibiting linearity in the range from 0.3 to 2 mu g.mL(-1) (Absorbance=0.18068 x [LPSF/AC04 mu g.mL(-1)] + 0.00348), (r(2)=0.9995). The limits of detection and quantification were 0.047 mu g.mL(-1) and 0.143 mu g.mL(-1), respectively. The method was accurate, precise, reproducible and robust since all the samples analyzed had coefficient of variation of less than 5% and no statistically significant difference between theoretical and practical concentrations was detected. Thus, a rapid, simple, low cost and sensitive spectrophotometric method was developed and validated for determining the content of inclusion complex and liposomes containing LPSF/AC04.

引用

页码：183 / 191

页数：9

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