Doxycycline-encapsulated solid lipid nanoparticles as promising tool against Brucella melitensis enclosed in macrophage: a pharmacodynamics study on J774A.1 cell line

被引：75

作者：

Hosseini, Seyed Mostafa ^{[1
]}

Abbasalipourkabir, Roghayyeh ^{[2
]}

Jalilian, Farid Azizi ^{[3
]}

Asl, Sara Soleimani ^{[4
]}

Farmany, Abbas ^{[5
]}

Roshanaei, Ghodratollah ^{[6
]}

Arabestani, Mohammad Reza ^{[1
,7
]}

机构：

[1] Hamadan Univ Med Sci, Fac Med, Dept Microbiol, Shahid Fahmideh St,Pk Mardome, Hamadan, Iran

[2] Hamadan Univ Med Sci, Dept Clin Biochem, Fac Med, Shahid Fahmideh St,Pk Mardome, Hamadan, Iran

[3] Hamadan Univ Med Sci, Dept Virol, Fac Med, Shahid Fahmideh St,Pk Mardome, Hamadan, Iran

[4] Hamadan Univ Med Sci, Dept Anat Sci, Fac Med, Shahid Fahmideh St,Pk Mardome, Hamadan, Iran

[5] Hamadan Univ Med Sci, Sch Dent, Dent Res Ctr, Shahid Fahmideh St,Pk Mardome, Hamadan, Iran

[6] Hamadan Univ Med Sci, Sch Hlth, Dept Biostat, Shahid Fahmideh St,Pk Mardome, Hamadan, Iran

[7] Hamadan Univ Med Sci, Brucellosis Res Ctr, Shahid Fahmideh St,Pk Mardome, Hamadan, Iran

来源：

ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL | 2019年 / 8卷 / 1期

关键词：

Brucella melitensis; Doxycycline; Solid lipid nanoparticle; Relapse; DELIVERY; SLN; FORMULATION;

D O I：

10.1186/s13756-019-0504-8

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

BackgroundBrucellosis is a zoonotic disease caused by Brucella species. It has been estimated that more than 500,000 new cases of Brucellosis occur annually all around the world. Relapse of the disease is one of the most important challenges. The most important reason for the relapse of brucellosis is the survival of the bacteria inside the macrophages, which makes them safe from the immune system and disrupts drug delivery mechanism.ObjectivesThe present study was performed to assess the effects of Doxycycline-loaded Solid Lipid Nanoparticles (DOX-SLN) on the Brucella melitensis inside macrophages.MethodsDOX-SLN was prepared using double emulsion method. The technological characterization of DOX-SLN, including particle size, zeta potential, polydispersity index (PDI), drug loading and encapsulation efficiency were used. Fourier-transform infrared spectroscopy (FTIR) and Differential scanning calorimetry (DSC) were used to assess the interactions between Nanoparticles (NPs) components and crystalline form of doxycycline. Moreover, the effect of DOX-SLN on the bacteria were compared with that of the doxycycline using various methods, including well diffusion, Minimum Inhibitory Concentration (MIC), and investigation of their effects on murine macrophage-like cells cell line J774A.1.ResultsThe means of particle size, zeta potential, PDI, drug loading and encapsulation efficiency were 29934nm, -28.7 +/- 3.2mV, 0.29 +/- 0.027, 11.2 +/- 1.3%, and 94.9 +/- 3.2%, respectively. The morphology of NPs were spherical with a smooth surface. No chemical reaction was occurred between the components. Doxycycline was located within NP matrix in its molecular form. The DOX-SLN significantly decreased the microbial loading within macrophages (3.5 Log) in comparison with the free doxycycline.Conclusions Since the DOX-SLN showed better effects on B. melitensis enclosed in macrophages than the free doxycycline, it is recommended to use it for treating brucellosis and preventing relapse.

引用

页数：12

共 35 条

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