FHL2 interacts with CALM and is highly expressed in acute erythroid leukemia

被引：9
作者：
Pasalic, Z. [2 ]
Greif, P. A. [2 ]
Jurinovic, V. [3 ]
Mulaw, M. [2 ]
Kakadia, P. M.
Tizazu, B. [2 ]
Froehlich-Archangelo, L. [2 ]
Krause, A. [2 ]
Bohlander, S. K. [1 ,2 ]
机构：
[1] Univ Munich, Univ Munich Hosp Grosshadern, Dept Med 3, Klinikum Grosshadern, D-81377 Munich, Germany
[2] Helmholtz Zentrum Munich, Natl Res Ctr Environm Hlth, Clin Cooperat Grp Leukemia, Munich, Germany
[3] Univ Munich, Inst Med Informat Biometry & Epidemiol, D-81377 Munich, Germany
来源：
BLOOD CANCER JOURNAL | 2011年 / 1卷
关键词：
CALM; AF10; FHL2; ACUTE MYELOID-LEUKEMIA; LIM-ONLY PROTEIN; ZINC-FINGER PROTEIN; PROMYELOCYTIC LEUKEMIA; SUBCELLULAR-LOCALIZATION; CELL-DIFFERENTIATION; CYTOPLASMIC DOMAIN; ANDROGEN RECEPTOR; CALM/AF10; FUSION; BETA-CATENIN;
D O I：
10.1038/bcj.2011.40
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The t(10;11)(p13;q14) translocation results in the fusion of the CALM (clathrin assembly lymphoid myeloid leukemia protein) and AF10 genes. This translocation is observed in acute myeloblastic leukemia (AML M6), acute lymphoblastic leukemia (ALL) and malignant lymphoma. Using a yeast two-hybrid screen, the four and a half LIM domain protein 2 (FHL2) was identified as a CALM interacting protein. Recently, high expression of FHL2 in breast, gastric, colon, lung as well as in prostate cancer was shown to be associated with an adverse prognosis. The interaction between CALM and FHL2 was confirmed by glutathione S-transferase-pulldown assay and co-immunoprecipitation experiments. The FHL2 interaction domain of CALM was mapped to amino acids 294-335 of CALM. The transcriptional activation capacity of FHL2 was reduced by CALM, but not by CALM/AF10, which suggests that regulation of FHL2 by CALM might be disturbed in CALM/AF10-positive leukemia. Extremely high expression of FHL2 was seen in acute erythroid leukemia (AML M6). FHL2 was also highly expressed in chronic myeloid leukemia and in AML with complex aberrant karyotype. These results suggest that FHL2 may play an important role in leukemogenesis, especially in the case of AML M6.
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页码：e42 / e42
页数：11
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