DNA methyltransferase BmDnmt1 and BmDnmt2 in silkworm (Bombyx mori) and the regulation of silkworm embryonic development

被引：12

作者：

Li, Bing ^{[1
,2
,3
]}

Hu, Pei ^{[1
,2
]}

Zhang, Shang-Zhi ^{[1
,2
]}

Toufeeq, Shahzad ^{[1
,2
]}

Wang, Jie ^{[1
,2
]}

Zhao, Kang ^{[1
,2
]}

Xu, Xin ^{[1
,2
]}

Xu, Jia-Ping ^{[1
,2
]}

Huang, Shou-Jun ^{[1
,2
]}

机构：

[1] Anhui Agr Univ, Sch Life Sci, Hefei 230036, Anhui, Peoples R China

[2] Anhui Int Joint Res & Dev Ctr Sericulture Resourc, Hefei, Anhui, Peoples R China

[3] Anhui Acad Agr Sci, Inst Sericulture, Hefei, Anhui, Peoples R China

来源：

ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY | 2019年 / 100卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Bombyx mori; diapause; DNA methylation; embryonic development; gene expression; METHYLATION; DIAPAUSE; MAINTENANCE; TERMINATION; EXPRESSION; INSIGHTS; EGG;

D O I：

10.1002/arch.21529

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA methylation is one of the most widespread epigenetic marks and has been linked to insect development, especially influencing embryonic development. However, the regulation of DNA methylation in silkworm embryonic development and diapause remain to investigate. In this study, reverse-transcription quantitative polymerase chain reaction was performed to identify the expression level of Bombyx mori DNA methyltransferases (BmDNMTs) 1 and 2 (BmDnmt1 and BmDnmt2) in different tissues, different embryonic developmental stages, and different strains of the silkworm. The results showed that BmDNMTs were the most highly expressed during embryonic development, especially at early embryonic stages. In particular, the expression of BmDNMTs was significantly upregulated in diapause-terminated eggs by HCl treatment. Moreover, tissue distribution showed that BmDnmt2 was highly expressed in testis and ovary, and BmDnmt1 was highly expressed in testis. This study contributes to understanding the correlation of DNA methylation occurs with embryogenesis and gametogenesis in insect, meanwhile, it provides a research orientation to further analyze the role of DNA methylation in diapause initiation and termination in insect embryonic development.

引用

页数：12

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