A Simple and Applicable Method for Human Platelet Lysate Preparation Using Citrate Blood

被引：1

作者：

Khongjaroensakun, Narin ^{[1
]}

Paisooksantivatana, Karan ^{[1
]}

Santiwatana, Suttikarn ^{[2
,3
]}

Tawonsawatruk, Tulyapruek ^{[4
]}

Kusolthammarat, Kantarat ^{[1
,5
]}

Kadegasem, Praguywan ^{[2
]}

Tangbubpha, Noppawan ^{[2
]}

Chantaraamporn, Juthamard ^{[2
]}

Chuansumrit, Ampaiwan ^{[2
]}

机构：

[1] Mahidol Univ, Fac Med, Dept Pathol, Ramathibodi Hosp, Bangkok, Thailand

[2] Mahidol Univ, Ramathibodi Hosp, Fac Med, Dept Pediat, Bangkok, Thailand

[3] Vejthani Hosp, Bangkok, Thailand

[4] Mahidol Univ, Fac Med, Dept Orthoped, Ramathibodi Hosp, Bangkok, Thailand

[5] Prince Songkla Univ, Dept Pathol, Hat Yai, Thailand

来源：

LABORATORY MEDICINE | 2022年 / 53卷 / 05期

关键词：

GROWTH-FACTORS; RICH PLASMA;

D O I：

10.1093/labmed/lmab116

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Objectives To determine and compare the platelet growth factors in human platelet lysate (HPL) prepared from citrated whole blood, with final centrifugations at 4(o)C and 25(o)C. Methods We collected specimens of citrated whole blood from 27 healthy volunteers. The platelet-rich plasma (PRP) was separated to prepare the HPL, which was further divided into 2 portions for the final centrifugation, at 4(o)C and 25(o)C, respectively. Platelet growth factors were measured and compared between the 2 groups. Results All platelet growth factors were higher than those in PRP prepared from citrated whole blood. Moreover, the final centrifugation at 25(o)C resulted in noninferiority of platelet-growth-factor level. Conclusion This study provided a simple method for small-volume of HPL preparation using only 10-15 mL of citrated whole blood. Further, the entire process of centrifugation can be performed at room temperature of 25(o)C, which is more applicable than lower temperatures for other laboratories.

引用

页码：E109 / E112

页数：4

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