The chemical genomic portrait of yeast: Uncovering a phenotype for all genes

被引：736

作者：

Hillenmeyer, Maureen E. ^{[1
,2
]}

Fung, Eula ^{[1
]}

Wildenhain, Jan ^{[3
]}

Pierce, Sarah E. ^{[1
,4
]}

Hoon, Shawn ^{[1
,4
]}

Lee, William ^{[1
,4
]}

Proctor, Michael ^{[1
]}

St Onge, Robert P. ^{[1
]}

Tyers, Mike ^{[3
,5
]}

Koller, Daphne ^{[6
]}

Altman, Russ B. ^{[2
,4
]}

Davis, Ronald W. ^{[2
,4
]}

Nislow, Corey ^{[5
,7
]}

Giaever, Guri ^{[5
,7
,8
]}

机构：

[1] Stanford Univ, Stanford Genome Technol Ctr, Palo Alto, CA 94304 USA

[2] Stanford Univ, Program Biomed Informat, Palo Alto, CA 94304 USA

[3] Univ Toronto, Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada

[4] Stanford Univ, Dept Genet, Palo Alto, CA 94304 USA

[5] Univ Toronto, Dept Mol Genet, Toronto, ON M5S 1A8, Canada

[6] Stanford Univ, Dept Comp Sci, Stanford, CA 94305 USA

[7] Univ Toronto, Donelley Ctr Cellular & Biomol Res, Toronto, ON M5S 3E1, Canada

[8] Univ Toronto, Dept Pharmaceut Sci, Toronto, ON M5S 3M2, Canada

来源：

SCIENCE | 2008年 / 320卷 / 5874期

关键词：

D O I：

10.1126/science.1150021

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Genetics aims to understand the relation between genotype and phenotype. However, because complete deletion of most yeast genes (similar to 80%) has no obvious phenotypic consequence in rich medium, it is difficult to study their functions. To uncover phenotypes for this nonessential fraction of the genome, we performed 1144 chemical genomic assays on the yeast whole- genome heterozygous and homozygous deletion collections and quantified the growth fitness of each deletion strain in the presence of chemical or environmental stress conditions. We found that 97% of gene deletions exhibited a measurable growth phenotype, suggesting that nearly all genes are essential for optimal growth in at least one condition.

引用

页码：362 / 365

页数：4

共 28 条

[1] A proteomic analysis of lysosomal integral membrane proteins reveals the diverse composition of the organelle [J].

Bagshaw, RD ;

Mahuran, DJ ;

Callahan, JW .

MOLECULAR & CELLULAR PROTEOMICS, 2005, 4 (02) :133-143

[2] Pharmacogenomic analysis: Correlating molecular substructure classes with microarray gene expression data [J].

Blower P.E. ;

Yang C. ;

Fligner M.A. ;

Verducci J.S. ;

Yu L. ;

Richman S. ;

Weinstein J.N. .

The Pharmacogenomics Journal, 2002, 2 (4) :259-271

[3] GO::TermFinder - open source software for accessing Gene Ontology information and finding significantly enriched Gene Ontology terms associated with a list of genes [J].

Boyle, EI ;

Weng, SA ;

Gollub, J ;

Jin, H ;

Botstein, D ;

Cherry, JM ;

Sherlock, G .

BIOINFORMATICS, 2004, 20 (18) :3710-3715

[4] Transcriptional networks: reverse-engineering gene regulation on a global scale [J].

Chua, G ;

Robinson, MD ;

Morris, Q ;

Hughes, TR .

CURRENT OPINION IN MICROBIOLOGY, 2004, 7 (06) :638-646

[5] A high-resolution map of transcription in the yeast genome [J].

David, L ;

Huber, W ;

Granovskaia, M ;

Toedling, J ;

Palm, CJ ;

Bofkin, L ;

Jones, T ;

Davis, RW ;

Steinmetz, LM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (14) :5320-5325

[6] Mechanisms of haploinsufficiency revealed by genome-wide profiling in yeast [J].

Deutschbauer, AM ;

Jaramillo, DF ;

Proctor, M ;

Kumm, J ;

Hillenmeyer, ME ;

Davis, RW ;

Nislow, C ;

Giaever, G .

GENETICS, 2005, 169 (04) :1915-1925

[7] Multiple knockout analysis of genetic robustness in the yeast metabolic network [J].

Deutscher, David ;

Meilijson, Isaac ;

Kupiec, Martin ;

Ruppin, Eytan .

NATURE GENETICS, 2006, 38 (09) :993-998

[8]

DUDLEY AM, 2005, MOL SYSTEMS BIOL, V1

[9] The yeast multidrug transporter Pdr5 of the plasma membrane is ubiquitinated prior to endocytosis and degradation in the vacuole [J].

Egner, R ;

Kuchler, K .

FEBS LETTERS, 1996, 378 (02) :177-181

[10] Analysis of the tangled relationships between P-glycoprotein-mediated multidrug resistance and the lipid phase of the cell membrane [J].

Ferté, J .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2000, 267 (02) :277-294

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