Phylogenetic reconstruction based on Cytochrome b (Cytb) gene sequences reveals distinct genotypes within Colombian Trypanosoma cruzi I populations

被引：44
作者：
David Ramirez, Juan [1 ]
Clara Duque, Maria [1 ]
Guhl, Felipe [1 ]
机构：
[1] Univ Los Andes, Fac Ciencias, Ctr Invest Microbiol & Parasitol Trop CIMPAT, Bogota, Colombia
来源：
ACTA TROPICA | 2011年 / 119卷 / 01期
关键词：
Chagas disease; DTU; Trypanosoma cruzi; Transmission cycles; Cytb; Maximum likelihood; PRIMER-PCR; LINEAGE-I; REGIONS; IDENTIFICATION; HYBRIDIZATION; VARIABILITY; EXCHANGE; STOCKS;
D O I：
10.1016/j.actatropica.2011.04.009
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Chagas disease caused by Trypanosoma cruzi comprises an important problem of public health in the Americas. This parasite has been recently divided into six Discrete Typing Units (DTUs) due to its high genetic diversity. We sequenced the Cytochorme b (Cytb) gene of 70 T. cruzi I Colombian clones finding four genotypes related to transmission cycles of Chagas disease in Colombia and also to specific hosts of T. cruzi. The genotypes herein described based on Cytb gene sequences are in accordance with those found using the mini-exon gene and reveals once again the enormous genetic diversity at sub-DTU level evidenced in T. cruzi I. (C) 2011 Elsevier B.V. All rights reserved.
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页码：61 / 65
页数：5
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