Quantum dots as a novel immunofluorescent detection system for Cryptosporidium parvum and Giardia lamblia

被引：97
作者：
Zhu, L
Ang, S
Liu, WT
机构：
[1] Natl Univ Singapore, Dept Civil Engn, Singapore 117576, Singapore
[2] Natl Univ Singapore, Dept Mech Engn, Singapore 117576, Singapore
[3] Univ Arkansas, Dept Elect Engn, Fayetteville, AR 72701 USA
[4] Univ Arkansas, Dept Biol & Agr Engn, Fayetteville, AR 72701 USA
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2004年 / 70卷 / 01期
关键词：
D O I：
10.1128/AEM.70.1.597-598.2004
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Semiconductor quantum dot-conjugated antibodies were successfully developed to label Cryptosporidium parvum and Giardia lamblia. This novel fluorescence system exhibited superior photostability, gave 1.5- to 9-fold-higher signal-to-noise ratios than traditional organic dyes in detecting C parvum, and allowed dual-color detection for C. parvum and G. lamblia.
引用
收藏
页码：597 / 598
页数：2
相关论文
共 10 条
  • [1] Semiconductor nanocrystals as fluorescent biological labels
    Bruchez, M
    Moronne, M
    Gin, P
    Weiss, S
    Alivisatos, AP
    [J]. SCIENCE, 1998, 281 (5385) : 2013 - 2016
  • [2] Quantum dot bioconjugates for ultrasensitive nonisotopic detection
    Chan, WCW
    Nie, SM
    [J]. SCIENCE, 1998, 281 (5385) : 2016 - 2018
  • [3] Characterization of Cryptosporidium spp. -: recent developments and future needs
    Egyed, Z
    Sréter, T
    Széll, Z
    Varga, I
    [J]. VETERINARY PARASITOLOGY, 2003, 111 (2-3) : 103 - 114
  • [4] Epidemiology of Cryptosporidium:: transmission, detection and identification
    Fayer, R
    Morgan, U
    Upton, SJ
    [J]. INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2000, 30 (12-13) : 1305 - 1322
  • [5] Avidin: A natural bridge for quantum dot-antibody conjugates
    Goldman, ER
    Balighian, ED
    Mattoussi, H
    Kuno, MK
    Mauro, JM
    Tran, PT
    Anderson, GP
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (22) : 6378 - 6382
  • [6] Long-term multiple color imaging of live cells using quantum dot bioconjugates
    Jaiswal, JK
    Mattoussi, H
    Mauro, JM
    Simon, SM
    [J]. NATURE BIOTECHNOLOGY, 2003, 21 (01) : 47 - 51
  • [7] Cryptosporidium parvum and Cyclospora cayetanensis:: a review of laboratory methods for detection of these waterborne parasites
    Quintero-Betancourt, W
    Peele, ER
    Rose, JB
    [J]. JOURNAL OF MICROBIOLOGICAL METHODS, 2002, 49 (03) : 209 - 224
  • [8] Vesey G, 1997, CYTOMETRY, V29, P147, DOI 10.1002/(SICI)1097-0320(19971001)29:2<147::AID-CYTO7>3.0.CO
  • [9] 2-A
  • [10] Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots
    Wu, XY
    Liu, HJ
    Liu, JQ
    Haley, KN
    Treadway, JA
    Larson, JP
    Ge, NF
    Peale, F
    Bruchez, MP
    [J]. NATURE BIOTECHNOLOGY, 2003, 21 (01) : 41 - 46
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