DEVELOPMENT OF POLYMORPHIC MICROSATELLITE MARKERS FOR INCARVILLEA SINENSIS (BIGNONIACEAE)

被引：4

作者：

Yu, Hai-Yan ^{[1
]}

Jing-Gao ^{[1
]}

Luo, Yi-Bo ^{[1
]}

Bai, Wei-Ning ^{[1
]}

机构：

[1] Chinese Acad Sci, State Key Lab Systemat & Evolutionary Bot, Inst Bot, Beijing 100093, Peoples R China

来源：

AMERICAN JOURNAL OF BOTANY | 2011年 / 98卷 / 08期

关键词：

Incarvillea sinensis; microsatellite; polymorphism; self-fertilization; subcloning; PLANTS;

D O I：

10.3732/ajb.1100052

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Premise of the study: Microsatellite markers were developed for Incarvillea sinensis var. sinensis (Bignoniaceae), an annual herb endemic to Inner Mongolia, to study the degree to which delayed self-fertilization is favored. Methods and Results: Eight polymorphic primer sets were isolated and characterized in two Inner Mongolia populations of I. sinensis var. sinensis with a relatively simple and fast subcloning method. Numbers of alleles per locus ranged from 2 to 7, with observed and expected heterozygosities ranging from 0 to 0.261 and from 0 to 0.778, respectively. Conclusions: These markers will be useful for future studies of self-fertilization adaptability in I. sinensis var. sinensis.

引用

页码：E224 / E225

页数：2

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