Preventive Effect of Probiotics and α-Tocopherol on Ethanol-Induced Gastric Mucosal Injury in Rats

被引：15
作者：
Senol, Altug [1 ]
Isler, Mehmet [1 ]
Karahan, Aynur G. [4 ]
Kilic, Gulden B. [5 ]
Kuleasan, Hakan [4 ]
Kaya, Selcuk [2 ]
Keskin, Muharrem [1 ]
Goren, Ibrahim [1 ]
Saritas, Ulku [1 ]
Aridogan, Buket C. [2 ]
Delibas, Namik [3 ]
机构：
[1] Suleyman Demirel Univ, Dept Gastroenterol, Fac Med, TR-32200 Isparta, Turkey
[2] Suleyman Demirel Univ, Dept Microbiol, Fac Med, TR-32200 Isparta, Turkey
[3] Suleyman Demirel Univ, Dept Biochem, Fac Med, TR-32200 Isparta, Turkey
[4] Suleyman Demirel Univ, Fac Engn, Dept Food Engn, TR-32200 Isparta, Turkey
[5] Mehmet Akif Ersoy Univ, Burdur Vocat Sch Higher Educ, Dept Dairy Prod, Burdur, Turkey
来源：
JOURNAL OF MEDICINAL FOOD | 2011年 / 14卷 / 1-2期
关键词：
ethanol; gastric injury; probiotic; rat; alpha-tocopherol; LACTOBACILLUS-RHAMNOSUS GG; PALM VITAMIN-E; FREE-RADICALS; ESCHERICHIA-COLI; ISCHEMIA-REPERFUSION; ULCERATIVE-COLITIS; DAMAGE; INFLAMMATION; VSLNUMBER-3; SUPEROXIDE;
D O I：
10.1089/jmf.2010.0040
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
The protective effect of a probiotic mixture of 13 different bacteria and alpha-tocopherol on 98% ethanol-induced gastric mucosal injury was evaluated. Levels of gastric mucosal pro- and anti-inflammatory cytokines, malondialdehyde, and secretory immunglobulin A were measured. Rats were allocated into four groups: control, ethanol, probiotic, and alpha-tocopherol. The control and ethanol groups received skim milk for 14 days. Probiotic and alpha-tocopherol groups were administered probiotic mixture suspended in skim milk and 100 mg/kg alpha-tocopherol, respectively, by daily gavage for 14 days. On Day 15, gastric lesions were induced by administration of ethanol 98% (1 mL) to all rats except those in the control group. Probiotic, but not alpha-tocopherol, seemed to inhibit ethanol-induced gastric mucosal tumor necrosis factor-a, interferon-g, and interleukin-2 production (P>.05). Ethanol caused the elevation of mucosal interleukin-4 level (compared to the control, P<.05). Probiotic pretreatment significantly suppressed the ethanol-induced increase of gastric mucosal interleukin-4 levels. Pretreatment with either probiotic or alpha-tocopherol inhibited the ethanol-induced increase of mucosal malondialdehyde concentration (P<.01 and P<.05, respectively). Probiotic pretreatment enhanced the gastric mucosal secretory immunoglobulin A concentration (P<.001). In conclusion, probiotic mixture and alpha-tocopherol reduced ethanol-induced gastric mucosal lipid peroxidation, suggesting that they may be beneficial for gastric lesions induced by lower ethanol concentration.
引用
收藏
页码：173 / 179
页数：7
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