Adiponectin/adiponectin receptors mRNA expression profiles in chickens and their response to feed restriction

被引：8

作者：

Cai, Jiangxue ^{[1
]}

Hu, Qingmei ^{[1
]}

Lin, Hai ^{[1
]}

Zhao, Jingpeng ^{[1
]}

Jiao, Hongchao ^{[1
]}

Wang, Xiaojuan ^{[1
]}

机构：

[1] Shandong Agr Univ, Dept Anim Sci & Technol, Shandong Prov Key Lab Anim Biotechnol & Dis Contr, Tai An 271018, Shandong, Peoples R China

来源：

POULTRY SCIENCE | 2021年 / 100卷 / 12期

基金：

中国国家自然科学基金;

关键词：

broiler; lipid metabolism; adiponectin; fasting; ACTIVATED PROTEIN-KINASE; CALORIC RESTRICTION; GENE-EXPRESSION; ADIPONECTIN RECEPTORS; ADIPOSE-TISSUE; BLOOD-PRESSURE; CDNA CLONING; FAT; METABOLISM; STARVATION;

D O I：

10.1016/j.psj.2021.101480

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Adiponectin (ADPN) is related to fatty acid synthesis and oxidation in mammals. In chickens, the lipid metabolism, structure and sequence of ADPN are dif-ferent from that in mammals. The aim of this study was to determine the role of ADPN in broilers lipid metabolism by investigating the temporal and spatial expression pro-files of ADPN and its receptors, as well as their response to feed restriction. The results showed that the abdominal fat has the highest expression level, followed by the duode-num, glandular stomach, heart, hypothalamus, liver, and skeletal muscle. Broilers have high energy mobilization during their early stage of growth, in which the fat demand in the liver and muscles is high, thus the expression of ADPN and its receptor are also increased. To study the effects of feed restriction on ADPN and lipid metabolism,broilers were fasted for 12 h and refeed for 2 h. The results showed that fasting decreased the concentration of triglyc-eride (TG) (P < 0.05) and total cholesterol (TCHO) (P < 0.05) in plasma. The mRNA expression of ADPN in the liver (P < 0.05), breast (P < 0.05) and thigh (P < 0.05), and the mRNA expression of ADPNR1 in the liver (P < 0.05) and duodenum (P < 0.05) were significantly increased in the Fasted group. All above phenomena were recovered after refeeding, suggesting that feed restriction may promote the utilization of fatty acids in active metab-olism tissues through ADPN, to guarantee the energy homeostasis of the body. However, the AMP-activated protein kinase (AMPK) signaling pathway and hepatic lipid metabolism were not necessary to cause the above changes under this experimental condition.

引用

页数：14

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