Rapid Odorant Release in Mammalian Odour Binding Proteins Facilitates Their Temporal Coupling to Odorant Signals

被引：15

作者：

Borysik, Antoni J. ^{[1
]}

Briand, Loic ^{[2
,3
,4
]}

Taylor, Andrew J. ^{[1
]}

Scott, David J. ^{[1
]}

机构：

[1] Univ Nottingham, Sch Biosci, Loughborough LE12 5RD, England

[2] ENESAD, FLAVIC UMR1169, F-21000 Dijon, France

[3] Univ Bourgogne, FLAVIC UMR1169, F-21000 Dijon, France

[4] INRA, FLAVIC UMR1169, F-21000 Dijon, France

来源：

JOURNAL OF MOLECULAR BIOLOGY | 2010年 / 404卷 / 03期

基金：

英国生物技术与生命科学研究理事会;

关键词：

olfaction; odorant-binding proteins; kinetics; mass spectrometry; STRUCTURAL-CHARACTERIZATION; OLFACTORY DISCRIMINATION; RAT; BIOCOMPATIBILITY; CELLULOSE; MECHANISM; DYNAMICS; RECEPTOR; LIGAND;

D O I：

10.1016/j.jmb.2010.09.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have measured the effect of rat odorant-binding protein 1 on the rates of ligand uptake and liquid-to-air transfer rates with a set of defined odorous compounds. Comparison of observed rate constants (k(obs)) with data simulated over a wide range of different kinetic and thermodynamic regimes shows that the data do not agree with the previously held view of a slow off-rate regime (k(off) <0.0004 s(-1)). We propose that a rapid koff would be a necessary requirement for such a system, since slow odorant-release rates would result in significant decorrelation between the olfactory world and odour perception. (c) 2010 Elsevier Ltd. All rights reserved.

引用

页码：372 / 380

页数：9

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