Identification of metal-binding to proteins in seed samples using RF-HPLC-UV, GFAAS and MALDI-TOF-MS

被引：6
|
作者：
Rigueira, Leila M. B. [1 ]
Lana, Diogo A. P. D. [2 ]
dos Santos, Daniel M. [3 ]
Pimenta, Adriano M. [3 ]
Augusti, Rodinei [2 ]
Costa, Leticia M. [2 ]
机构：
[1] Univ Fed Vales Jequitinhonha & Mucuri, Inst Engn Ciencia & Tecnol, BR-39440000 Janauba, Brazil
[2] Univ Fed Minas Gerais, Dept Quim, BR-31270901 Belo Horizonte, MG, Brazil
[3] Univ Fed Minas Gerais, Dept Bioquim & Imunol, BR-31270901 Belo Horizonte, MG, Brazil
来源：
FOOD CHEMISTRY | 2016年 / 211卷
关键词：
Metals; Proteins; RP-HPLC-UV; GFAAS; MALDI-TOF-MS; SIZE-EXCLUSION CHROMATOGRAPHY; X-RAY-FLUORESCENCE; BRAZIL-NUT; ICP-MS; FRACTIONS; PLASMA; COPPER; ELECTROPHORESIS; METALLOPROTEINS; SPECTROMETRY;
D O I：
10.1016/j.foodchem.2016.05.061
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
An extraction procedure using Tris-HCl buffer solution was employed in order to extract water-soluble proteins from seed samples of oat, wheat and soybean. Initially, the total protein concentration was determined by the Bradford method in each solution, after the extraction procedure. The soybean sample showed a higher concentration of total protein compared to the others. The protein extracts obtained were separated by reverse-phase chromatography (RP-HPLC-UV). The protein fractions were collected and analyzed by graphite furnace atomic absorption spectrometry (GFAAS) and matrix-assisted laser desorption/ionization (MALDI-TOF-MS) for determination of Cu, Fe, Mn and Zn and identification of proteins, respectively. The combination of techniques such as RP-HPLC-UV, GFAAS and MALDI-TOF-MS allowed the identification of several proteins bound to metals present in the seed samples. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：910 / 915
页数：6
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