Evaluation of different selective media for enumeration of probiotic micro-organisms in combination with yogurt starter cultures in fermented milk

被引：20
作者：
Saccaro, Daniela Marques [1 ]
Hirota, Claudia Y. [1 ]
Tamime, Adnan Y.
de Oliveira, Marice Nogueira [1 ]
机构：
[1] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Tecnol Bioquim Farmaceut, Sao Paulo, Brazil
来源：
AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH | 2012年 / 6卷 / 10期
基金：
巴西圣保罗研究基金会;
关键词：
Selective media; probiotic; starter cultures; fermented milk; DELBRUECKII SSP BULGARICUS; ANIMALIS SUBSP LACTIS; LACTOBACILLUS-ACIDOPHILUS; BIFIDOBACTERIUM-ANIMALIS; STREPTOCOCCUS-THERMOPHILUS; BACTERIA; SURVIVAL; VIABILITY; COUNTS; CASEI;
D O I：
10.5897/AJMR11.117
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The aim of this study was to assess selective plating methodologies for the enumeration and identification of Streptococcus thermophilus, Lactobacillus delbrueckii ssp. bulgaricus, Lactobacillus acidophilus, Lactobacillus rhamnosus and Bifidobacterium animalis ssp. lactis in fermented milks. Seven agar media (MRS with added sorbitol, clindamycin or vancomycin, acidified MRS, RCA with added aniline blue and dicloxacilin, M17 and ST) were evaluated. The results showed that RCA dicloxacilin agar was suitable for the selective enumeration of B. animalis ssp. lactis in fermented milk. Either MRS (acidified) or M17 agar could be used for enumeration of L. delbrueckii ssp. bulgaricus and S. thermophilus, respectively. MRS media containing antibiotics were effective for the enumeration of the probiotic organisms (L. rhamnosus and L. acidophilus) inoculated in fermented milks.
引用
收藏
页码：2239 / 2245
页数：7
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