Time-resolved confocal fluorescence microscopy of porphyrins for phototherapy

被引：4
|
作者：
Spizzirri, PG
Hill, JS
Kahl, SB
Ghiggino, KP
机构：
[1] UNIV MELBOURNE, SCH CHEM, PARKVILLE, VIC 3052, AUSTRALIA
[2] ROYAL MELBOURNE HOSP, MELBOURNE NEUROSCI CTR, PARKVILLE, VIC 3052, AUSTRALIA
[3] UNIV CALIF SAN FRANCISCO, SCH PHARM, DEPT PHARMACEUT CHEM, SAN FRANCISCO, CA 94143 USA
来源：
LASERS IN MEDICAL SCIENCE | 1996年 / 11卷 / 04期
关键词：
fluorescence; time-resolved confocal microscopy; porphyrins; fluorescence polarization; photodynamic therapy;
D O I：
10.1007/BF02134914
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
The application of a novel time-resolved confocal fluorescence microspectrometer to studies of the distribution and speciation of porphyrin photosensitizers in rat C6 cerebral glioma cells is described. The instrument combines a mode-locked argon ion laser excitation source with time-correlated single photon counting fluorescence detection and has sub-micron spatial and sub-nanosecond temporal resolution, The porphyrins studied were haematoporphyrin derivative (HpD), haematoporphyrin IX (HP), porphyrin c (Pc) and the tetrakiscarborane carboxylate ester of 2,4-(alpha, beta-dihydroxyethyl) deuteroporphyrin IX (BOPP), From the heterogeneous emission observed in vitro, assignments and spatial location of various porphyrin species are proposed.
引用
收藏
页码：237 / 246
页数：10
相关论文
共 50 条
  • [1] Time-resolved single molecule confocal fluorescence microscopy of cytochrome c
    Lee, M
    Lee, SF
    Tang, JY
    Hochstrasser, RM
    BIOPHYSICAL JOURNAL, 2004, 86 (01) : 475A - 475A
  • [2] Time-resolved fluorescence microscopy
    Suhling, K
    French, PMW
    Phillips, D
    PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 2005, 4 (01) : 13 - 22
  • [3] Time-resolved fluorescence microscopy
    Suhling, Klaus
    Tregidgo, Carolyn
    Sergent, Nicolas
    Levitt, James A.
    Pavlides, Alex
    Green, Mark
    Hungerford, Graham
    Rei, Ana
    Ferreira, M. Isabel C.
    ADVANCED PHOTON COUNTING TECHNIQUES II, 2007, 6771
  • [4] Time-resolved fluorescence microscopy
    Klaus Suhling
    Paul M. W. French
    David Phillips
    Photochemical & Photobiological Sciences, 2005, 4 : 13 - 22
  • [5] Time-resolved confocal fluorescence microscopy of trinitrobenzene-responsive organic nanofibers
    Carlo Giansante
    Alexandre G. L. Olive
    Christian Schäfer
    Guillaume Raffy
    André Del Guerzo
    Analytical and Bioanalytical Chemistry, 2010, 396 : 125 - 131
  • [6] Time-resolved confocal fluorescence microscopy of trinitrobenzene-responsive organic nanofibers
    Giansante, Carlo
    Olive, Alexandre G. L.
    Schafer, Christian
    Raffy, Guillaume
    Del Guerzo, Andre
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2010, 396 (01) : 125 - 131
  • [7] Time-resolved 3D confocal fluorescence microscopy on living cells
    Schellenberg, M.
    Peev, E.
    Kloster, M.
    Napier, J.
    Neu, W.
    LASER APPLICATIONS IN LIFE SCIENCES, 2010, 7376
  • [8] Fluorescence Dynamics in the Endoplasmic Reticulum of a Live Cell: Time-Resolved Confocal Microscopy
    Ghosh, Shirsendu
    Nandi, Somen
    Ghosh, Catherine
    Bhattacharyya, Kankan
    CHEMPHYSCHEM, 2016, 17 (18) : 2818 - 2823
  • [9] Confocal time-resolved fluorescence anisotropy imaging
    Bader, Arjen N.
    Hofman, Erik G.
    Henegouwen, Paul van Bergen en
    Gerritsen, Hans C.
    IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES V, 2007, 6441
  • [10] Platinum porphyrins as phosphorescent label for time-resolved microscopy
    deHaas, RR
    vanGijlswijk, RPM
    vanderTol, EB
    Zijlmans, HJMAA
    BakkerSchut, T
    Bonnet, J
    Verwoerd, NP
    Tanke, HJ
    JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1997, 45 (09) : 1279 - 1292
12345