Depth-Resolved Structural and Compositional Characterization of Ion-Implanted Polystyrene that Enables Direct Covalent Immobilization of Biomolecules

被引：25

作者：

Bilek, Marcela Milena Marie ^{[1
]}

Kondyurin, Alexey ^{[1
]}

Dekker, Stephen ^{[1
]}

Steel, Bradley Clifton ^{[1
]}

Wilhelm, Richard Arthur ^{[4
,5
]}

Heller, Rene ^{[4
]}

McKenzie, David Robert ^{[1
]}

Weiss, Anthony Steven ^{[6
,7
,8
]}

James, Michael ^{[2
,3
]}

Moeller, Wolfhard ^{[4
]}

机构：

[1] Univ Sydney, Sch Phys A28, Appl & Plasma Phys Lab, Sydney, NSW 2006, Australia

[2] Australian Nucl Sci & Technol Org, Bragg Inst, Kirrawee Dc, NSW 2232, Australia

[3] Australian Synchrotron, Clayton, Vic 3168, Australia

[4] Helmholtz Zentrum Dresden Rossendorf, Inst Ion Beam Phys & Mat Res, D-01328 Dresden, Germany

[5] Tech Univ Dresden, D-01069 Dresden, Germany

[6] Univ Sydney, Sch Mol Biosci, Sydney, NSW 2006, Australia

[7] Univ Sydney, Charles Perkins Ctr, Sydney, NSW 2006, Australia

[8] Univ Sydney, Bosch Inst, Sydney, NSW 2006, Australia

来源：

JOURNAL OF PHYSICAL CHEMISTRY C | 2015年 / 119卷 / 29期

基金：

澳大利亚研究理事会;

关键词：

X-RAY REFLECTIVITY; NEUTRON REFLECTOMETRY; POLYMER-FILMS; BEAM ANALYSIS; PLASMA; PROTEIN; TROPOELASTIN; SURFACES; ATTACHMENT; DEGRADATION;

D O I：

10.1021/acs.jpcc.5b05164

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A polystyrene film spun onto polished silicon substrates was implanted with argon ions using plasma immersion ion implantation (PHI) to activate its surface for single-step immobilization of biological molecules. The film was subsequently investigated by X-ray and neutron reflectometry, ultraviolet (UV) visible (vis) and Fourier transform infrared (FTIR) ellipsometry, FTIR and Raman spectroscopy, as well as nuclear reaction analysis to determine the structural and compositional transformations associated with the surface activation. The ion irradiation resulted in a significant densification of the carbon structure, which was accompanied by hydrogen loss. The density and hydrogen profiles in the modified surface layers were found to agree with the expected depths of ion implantation as calculated by the Stopping and Range of Ions in Matter (SRIM) software. The data demonstrate that the reduction in film thickness is due to ion-induced densification rather than the removal of material by etching. Characterization by FTIR, atomic force microscopy (AFM), ellipsometry, and X-ray reflectometry shows that polystyrene films modified in this way imniobilize dense layers of protein (tropoelastin) directly from solution. A substantial fraction of the immobilized protein layer remains after rigorous washing with sodium dodecyl sulfate solution, indicating that its immobilization is by covalent bonding.

引用

页码：16793 / 16803

页数：11

共 65 条

[1] Degradation of polystyrene thin films under d, He-4 and C-12 irradiation studied by ion beam analysis: Effects of energy loss, sample thickness and isotopic content [J].

Abel, F ;

Quillet, V ;

Schott, M .

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS, 1995, 105 (1-4) :86-90

[2] ION-BEAM-INDUCED HYDROGEN RELEASE FROM A-C-H - A BULK MOLECULAR RECOMBINATION MODEL [J].

ADEL, ME ;

AMIR, O ;

KALISH, R ;

FELDMAN, LC .

JOURNAL OF APPLIED PHYSICS, 1989, 66 (07) :3248-3251

[3] Optical properties of surface modified polypropylene by plasma immersion ion implantation technique [J].

Ahmed, Sk Faruque ;

Moon, Myoung-Woon ;

Kim, Chansoo ;

Jang, Yong-Jun ;

Han, Seonghee ;

Choi, Jin-Young ;

Park, Won-Woong ;

Lee, Kwang-Ryeol .

APPLIED PHYSICS LETTERS, 2010, 97 (08)

[4]

Anders A., 2000, Handbook of plasma immersion ion implantation and deposition, V8

[5] Shape of tropoelastin, the highly extensible protein that controls human tissue elasticity [J].

Baldock, Clair ;

Oberhauser, Andres F. ;

Ma, Liang ;

Lammie, Donna ;

Siegler, Veronique ;

Mithieux, Suzanne M. ;

Tu, Yidong ;

Chow, John Yuen Ho ;

Suleman, Farhana ;

Malfois, Marc ;

Rogers, Sarah ;

Guo, Liang ;

Irving, Thomas C. ;

Wess, Tim J. ;

Weiss, Anthony S. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (11) :4322-4327

[6] Plasma modified surfaces for covalent immobilization of functional biomolecules in the absence of chemical linkers: Towards better biosensors and a new generation of medical implants [J].

Bilek M.M. ;

McKenzie D.R. .

Biophysical Reviews, 2010, 2 (2) :55-65

[7] Free radical functionalization of surfaces to prevent adverse responses to biomedical devices [J].

Bilek, Marcela M. M. ;

Bax, Daniel V. ;

Kondyurin, Alexey ;

Yin, Yongbai ;

Nosworthy, Neil J. ;

Fisher, Keith ;

Waterhouse, Anna ;

Weiss, Anthony S. ;

dos Remedios, Cristobal G. ;

McKenzie, David R. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (35) :14405-14410

[8] Osteoblast interaction with DLC-coated Si substrates [J].

Chai, Feng ;

Mathis, Nicolas ;

Blanchemain, Nicolas ;

Meunier, Cathy ;

Hildebrand, Hartmut F. .

ACTA BIOMATERIALIA, 2008, 4 (05) :1369-1381

[9]

Chow J., 2006, THESIS U SYDNEY SYDN

[10] A model for ion-irradiation induced hydrogen loss from organic materials [J].

deJong, MP ;

Maas, AJH ;

vanIjzendoorn, LJ ;

Klein, SS ;

deVoigt, MJA .

JOURNAL OF APPLIED PHYSICS, 1997, 82 (03) :1058-1064

← 1 2 3 4 5 6 7 →